Publications

2022:

2021:

2020:

  • Engineering orthogonal methyltransferases to create alternative bioalkylation pathways. A.  J. Herbert, S. A. Shepherd, V. A. Cronin, M. R. Bennett, R. Sung & J Micklefield* Angew. Chem. Int. Ed. 2020, 59, 2–9. (https://doi.org/10.1002/anie.202004963); Also see Nature Chemistry News & Views (https://rdcu.be/cmde1).
  • Engineering Escherichia coli towards de novo production of gatekeeper (2S)-flavanones: naringenin, pinocembrin, eriodictyol and homoeriodictyol. Dunstan, M., Robinson, C., Jervis, A., Yan, C., Carbonell, P., Hollywood, K., Currin, A., Swainston, N., Le Feuvre, R., Micklefield, J., Faulon, J-L., Breitling, R., Turner, N., Takano, E. & Scrutton, N. Synthetic Biology 2020, in press.(https://doi.org/10.1093/synbio/ysaa012)
  • Rapid prototyping of biosynthetic monomers production for enhanced biomanufacturing of materials. C. J. Robinson, P. Carbonell, A. J. Jervis, C. Yan, K. A. Hollywood, M. S. Dunstan, A. Currin, R. Spiess, N. Swainston, S. Taylor, P. Mulherin, N. Matthews, K. Malone, R. Le Feuvre, P. Shapira, P. Barran, N. J. Turner, J. Micklefield, R. Breitling, E. Takano, N. S. Scrutton Metabolic Engineering 2020, 60, 168–182. (https://doi.org/10.1016/j.ymben.2020.04.008).
  • Editorial overview: Biocatalysis and biotransformations. D. J. Campopiano & J.  Micklefield Current Opinion in Chemical Biology,  2020, 55, A1-A3 (https://doi.org/10.1016/j.cbpa.2020.04.019)
  • Harnessing and engineering amide bond forming ligases for the synthesis of amides. M. Winn, S. Richardson, D. J. Campopiano & J.  Micklefield* Current Opinion in Chemical Biology 2020, 55, 77–85 (https://doi.org/10.1016/j.cbpa.2019.12.004)

2019:

  • Engineering Enzymatic Assembly Lines to Produce New Antibiotics. K. A. J. Bozhüyük, J. Micklefield, B. Wilkinson Current Opinion in Microbiology 2019, 51, 88-96. (https://doi.org/10.1016/j.mib.2019.10.007)
  • Streamlined recycling of S-adenosylmethione (News & Views). J. Micklefield Nature Catalysis 2019, 2, 644-645. (https://doi.org/10.1038/s41929-019-0323-6)
  • Assembling a plug-and-play production line for combinatorial biosynthesis of aromatic polyketides in Escherichia coli, M. Cummings, A. Peters, G. Whitehead, B. K. Menon, J. Micklefield, S. Webb & E. Takano, PLOS Biology 2019, 17, e3000347 (https://doi.org/10.1371/journal.pbio.3000347)

2018

  • A vitamin K-dependent carboxylase is involved in antibiotic biosynthesis. B. J. C. Law, Y. Zhuo, D. Francis, M. Winn, Y. Zhang, M. Samborskyy, A. Murphy, L. Ren, P. F. Leadlay & J. Micklefield. Nature Catalysis 2018, 1, 977–984 (rdcu.be/bbZ99 ). Also see Nature Catalysis News & Views (https://rdcu.be/bdd6j) and Nature Research Community News (https://go.nature.com/2r4Smbu)
  • Structure and Biocatalytic Scope of Coclaurine N-Methyltransferase. M. R. Bennett, M. L. Thompson, S. A. Shepherd, M. S. Dunstan, A. J. Herbert, D. R. M. Smith, V. A. Cronin, B. R. K. Menon, C. Levy & J. Micklefield Angew. Chem. Int. Ed. 2018, 57, 10600-10604. (http://dx.doi.org/10.1002/anie.201805060)
  • De novo Biosynthesis of ‘Non-Natural’ Thaxtomin Phytotoxins. M. Winn, D. Francis & J. Micklefield  Angew. Chem. Int. Ed. 2018, 57, 6830–6833 (http://dx.doi.org/10.1002/anie.201801525)
  • The Cycloaspeptides: Uncovering a new model for methylated nonribosomal peptide biosynthesis. K. M. J. de Mattos-Shipley, C. Greco, D. M. Heard, G. Hough, N. P. Mulholland, J. L. Vincent, J. Micklefield, T. J. Simpson, C. L. Willis, R. J. Cox and A. M. Bailey Chem. Sci. 2018, 9, 4109-4117 (http://dx.doi.org/10.1039/C8SC00717A)
  • Development of Halogenase Enzymes for Use in Synthesis J. Latham, E. Brandenburger, S. A. Shepherd, B. R. K. Menon and J.Micklefield Chem. Rev 2018, 118, 232-269;  (http://dx.doi.org/10.1021/acs.chemrev.7b00032)
  • An automated Design-Build-Test-Learn pipeline for enhances microbial production of fine chemicals Carbonell, P. et al Communication Biology 2018, 1, 66 (http://dx.doi.org/10.1038/s42003-018-0076-9)

2017

  • RadH: A Versatile Halogenase for Integration into Synthetic Pathways. B. R. K. Menon, E. Brandenburger, H. H. Sharif, U. Klemstein, S.A. Shepherd, M. F. Greaney, and J. Micklefield  Angew. Chem. Int. Ed. 2017, 56, 11841 –11845 (http://dx.doi.org/10.1002/anie.201706342)
  • From multi-step Enzyme Monitoring to Whole-Cell Biotransformations: development of real-time UVRR spectroscopy. H. Fisk, Y. Xu, C.Westley, N. Turner, J. Micklefield, R. Goodacre. Analytical Chemistry 2017, 89, 12527-12532 (http://dx.doi.org/10.1021/acs.analchem.7b03742)  
  • Development of Halogenase Enzymes for use in Synthesis. Jonathan Latham, Eileen Brandenburger, Sarah A. Shepherd, Binuraj R. K. Menon and Jason Micklefield. Chem. Rev. 2017, in press (DOI: 10.1021/acs.chemrev.7b00032
  • Real-time monitoring of enzyme-catalysed reactions using deep UV resonance Raman spectroscopy C. Westley, H. Fisk, Y. Xu, K. A. Hollywood,  A. J. Carnell, J. Micklefield, N. J. Turner, R. Goodacre Chem. Eur. J., 2017, 23, 6983–6987 (http://dx.doi.org/10.1002/chem.201701388)
  • Recent Advances in Methyltransferase Biocatalysis. M.R. Bennett, S.A. Shepherd, V.A. Cronin, J. Micklefield Current Opinion in Chemical Biology. 2017, 37, 97-106. (http://dx.doi.org/10.1016/j.cbpa.2017.01.020)
  • An Engineered Tryptophan Synthase Opens New Enzymatic Pathways to β-Methyltryptophan and Derivatives. Daniel Francis, Michael Winn, Jonathan Latham, Michael F Greaney and Jason Micklefield. ChemBioChem. 2017, 18, 382-386 (http://dx.doi.org/10.1002/cbic.201600471)

2016

  • Integrated Catalysis Opens New Arylation Pathways via Regiodivergent Enzymatic C-H Activation. J. Latham, J.-M. Henry, H. H. Sharif, B. R. K. Menon, S. A. Shepherd,  M. F. Greaney, J.Micklefield. Nature Commun. 2016, 7, 11873 (http://dx.doi.org/10.1038/NCOMMS11873)
  • Rewiring Riboswitches to Create New Genetic Circuits in Bacteria. C.J. Robinson, D. Medina-Stacey, M.-C. Wu, H.A. Vincent, J. Micklefield Methods in Enzymology 2016, 575, 319–348.(http://dx.doi.org/10.1016/bs.mie.2016.02.022)
  • A flavin-dependent decarboxylase-dehydrogenase-monooxygenase assembles the warhead of α, β-epoxyketone proteasome inhibitors. D. Zabala; J. W. Cartwright; D. M. Roberts; B. J. C. Law; L. Song; M. Samborskyy; P. F. Leadlay; J. Micklefield; G. L. Challis J. Am. Chem. Soc. 2016, 138: 4342–4345 (http://dx.doi.org/10.1021/jacs.6b01619)
  • An Enzyme Cascade for Selective Modification of Tyrosine
    Residues in Structurally Diverse Peptides and Proteins.     A.-W. Struck, M. R. Bennett, S. A. Shepherd, B. J. C. Law, Y. Zhuo, L. S. Wong, J. Micklefield J. Am. Chem. Soc. 2016, 138, 3038-3045  (http://dx.doi.org/10.1021/jacs.5b10928) 
  • A Structure-Guided Switch in the Regioselectivity of a Tryptophan Halogenase. S. A. Shepherd, B. R. K. Menon, H. Fisk, A.-W. Struck, C. Levy, D. Leys, J. Micklefield ChemBioChem 2016, 17, 821–824  (http://dx.doi.org/10.1002/cbic.201600051)
  • Recent Advances in Engineering Non-Ribosomal Peptide Assembly Lines. M. Winn, J. Fyans, Y. Zhuo, J. Micklefield Nat. Prod. Rep. 2016, 33, 317-347  (http://dx.doi.org/10.1039/C5NP00099H)
  • Effects of Active Site Modification and Quaternary Structure on the Regioselectivity of Catechol-O-Methyltransferase. B. J. C. Law, M. R. Bennett, M. L. Thompson, C. Levy, S. A. Shepherd, D. Leys, and J. Micklefield Angew. Chem. Int. Ed. 2016, 55: 2683-2687. (http://dx.doi.org/10.1002/anie.201508287)
  • Dual transcriptional-translational cascade permits cellular level tuneable expression control. R. Morra, J. Shankar, C.J.Robinson, L. Butler, S. Halliwell, S. Hay, J. Micklefield, N. Dixon Nucleic Acid Res. 2016, 44, e21 (http://dx.doi.org/10.1093/nar/gkv912) 
  • SYNBIOCHEM Synthetic Biology Research Centre, Manchester – A UK foundry for fine and speciality chemicals production. Le Feuvre R. A.; Carbonell P.; Currin, A.; Dunstan, M.; Fellows, D.; Jervis, A. J.; Rattray, N. J. W.; Robinson, C. J.; Swainston, N.; Vinaixa, M.; Williams, A.; Yan, C.; Barran, P.; Breitling, R.; Chen, G. G.; Faulon, J. L.; Goble, C.; Goodacre, R.; Kell, D. B.; Micklefield, J.; Scrutton, N. S.; Shapira, P; Takano, E.; Turner, N. J.; Synthetic and Systems Biotechnology 1 2016, 271e275 (http://dx.doi.org/10.1016/j.synbio.2016.07.001)
  • SYNBIOCHEM-a SynBio foundry for the biosynthesis and sustainable production of fine and speciality chemicals. Carbonell P , Currin A , Dunstan M , Fellows D , Jervis A , Rattray NJ , Robinson CJ , Swainston N , Vinaixa M , Williams A , Yan C , Barran P , Breitling R , Chen GG , Faulon JL , Goble C , Goodacre R , Kell DB , Feuvre RL , Micklefield J , Scrutton NS , Shapira P , Takano E , Turner  Biochemical Society Transactions 2016, 44, 675-677 (http://dx.doi.org/10.1042/BST20160009)
  • Changing the Selectivity of Biocatalysts. M. Campbell, J. Micklefield ChemViews, 2016, 05, April  (http://dx.doi.org/10.1002/chemv.201600027) (Magazine article)
  • Structure and Biocatalytic Scope of Thermophillic Flavin-Dependent Halogenase and Flavin Reductase Enzymes B. R. K. Menon, J. Latham, M. S. Dunstan, E. Brandenburger, U. Klemstein, S. A. Shepherd, C. Karthikeyan, M. F. Greaney, D. Leys and J. Micklefield* Org. Biomol. Chem. 2016, 14, 9354–9361. (http://dx.doi.org/10.1039/C6OB01861K)

 

2015

  • Rational Re-engineering of the Transcriptional Silencing PreQ1 Riboswitch. M.-C. Wu, P. T. Lowe, C. J. Robinson, H. A. Vincent, N. Dixon, J. Leigh,  J. Micklefield J. Am. Chem. Soc. 2015, 137, 9015-9021 (http://dx.doi.org/10.1021/jacs.5b03405)
  • Extending the Biocatalytic Scope of Regiocomplementary Flavin-Dependent Halogenase Enzymes. S. A. Shepherd, C. Karthikeyan, J. Latham, A.-W. Struck, M. L. Thompson, B. Menon, M. Styles, C. Levy, D. Leys and J. Micklefield Chemical Science 2015, 6, 3454-3460 (http://dx.doi.org/10.1039/C5SC00913H)
  • Engineered Biosynthesis of Enduracidin Lipogyclopeptide Antibiotics using the Ramoplanin Mannosyltransferase Ram29. M.-C. Wu, M. Q. Styles, B. J. C. Law, A. W. Struck, L. Nunns and J. Micklefield Microbiology 2015, 161: 1338-1347 DOI:10.1099/mic.0.000095
  • Functional Exchangeability of Oxidase and Dehydrogenase Reactions in the Biosynthesis of Hydroxyphenylglycine, a Nonribosomal Peptide Building Block. V. Diez, M. Loznik, S. Taylor, M. Winn, N. J. W. Rattray, H. Podmore, J. Micklefield, R. Goodacre, M. H. Medema, U. Müller, R. Bovenberg, D. B. Janssenand E. Takano ACS synthetic Biology 2015, 4, 796-807.  DOI:10.1021/sb500368w
  • Site-specific bioalkylation of Rapamycin by the RapM 16-O-methyltransferase. B. J. C. Law, A.-W. Struck, M. R. Bennett, B. Wilkinson and J. Micklefield Chemical Science 2015, 6, 2885-2892 DOI:10.1039/C5SC00164A
  • Enzymatic Enantioselective Decarboxylative Protonation of Heteroaryl Malonates. R. Lewin, M. Goodall, M. L.Thompson, J. Leigh, M. Breuer, K. Baldenius and J. Micklefield Chem. Eur. J., 2015, 17, 6557-6563 DOI:10.1002/chem.201406014
  • Minimum Information about a Biosynthetic Gene cluster (MIBiG) specification. M. H. Medema et al. Nature Chem. Biol. 2015, 11, 625–631. (http://dx.doi.org/10.1038/nchembio.1890)

2014

  • Enzymatic Carboxylation and Decarboxylation. R. Lewin, M. Thompson and J. Micklefield Science of Synthesis: Biocatalysis in Organic Synthesis, 2014, 2, 133-157
  • A High-Throughput Assay for Arylamine Halogenation Based on a Peroxidase-Mediated Quinone-Amine Coupling with Applications in the Screening of Enzymatic Halogenations. J. Hosford, S.A. Shepherd, J. Micklefield, L. S. Wong Chem. Eur. J., 2014, 20, 16759-16763 DOI:10.1002/chem.201403953
  • Modular Riboswitch Toolsets for Synthetic Genetic Control in Diverse Bacterial Species. C. J. Robinson, H. A. Vincent, M.-C. Wu, P. T. Lowe, M. S. Dunstan,D. Leys, and J. Micklefield J. Am. Chem. Soc. 2014, 136, 10615-10624. DOI:10.1021/ja502873j
  • Generation of Orthogonally Selective Bacterial Riboswitches by Targeted Mutagenesis and in vivo Screening. H. Vincent, C. Robinson, M.-C Wu, N. Dixon and J. Micklefield Methods in Molecular Biology 2014, 1111, 107-129  DOI:10.1007/978-1-62703-755-6_8

2013

  • Nucleic acids: new life, new materials. M. J. Gait, M. Komiyama, N. C. Seeman, O. Seitz, J. Micklefield and D. R. Liu Org. Biomol. Chem. 2013, 11, 2058-2059 (commentary). DOI:10.1039/C3OB90017G

2012

  • Probing riboswitch-ligand interactions using thiamine pyrophosphate analogues. Chen L, Cressina E, Dixon N, Erixon K, Agyei-Owusu K, Micklefield J, Smith A, Abell C, Leeper F.  Org. Biomol. Chem., 2012, 10, 5924-5931. DOI:10.1039/c2ob07116a
  • Thermal Bifunctionality of Bacterial Phenylalanine Aminomutase and Ammonia Lyase Enzymes. Chesters C, Wilding M, Goodall M, Micklefield J.  Angew. Chem. Int. Ed. Engl., 2012, 51, 4344-4348.  DOI:10.1002/anie.201200669
  • Orthogonal riboswitches for tuneable coexpression in bacteria. Dixon N, Robinson C, Geerlings T, Duncan J, Drummond S, Micklefield J. Angew. Chem. Int. Ed. Engl., 2012, 51(15), 3620-4. DOI:10.1002/anie.201109106
  • Introduction of a non-natural amino Acid into a nonribosomal Peptide antibiotic by modification of adenylation domain specificity. Thirlway J, Lewis R, Nunns L, Al Nakeeb M, Styles M, Struck A, Smith C, Micklefield J.  Angew. Chem. Int. Ed. Engl., 2012, 51(29): DOI:10.1002/anie.201202043
  • Decarboxylases: Enzymatic enantioselective decarboxylative protonation and C-C bond formation. Wilding M, Goodall M, Micklefield J. in Comprehensive Chirality, H. Yamamoto & E. Carreira (Eds.). 2012, January: eScholarID:161723
  • A methodology for preparing nanostructured biomolecular interfaces with high enzymatic activity. Wong L, Karthikeyan C, Eichelsdoerfer D, Micklefield J, Mirkin C.  Nanoscale, 2012, 4(2), 659-66. DOI:10.1039/c1nr11443c
  • Bioengineering natural product biosynthetic pathways for therapeutic applications. Wu M, Law B, Wilkinson B, Micklefield J.  Curr. Opin. Biotechnol., in press. 2012, DOI:10.1016/j.copbio.2012.03.008

2011

  • Borrelidin modulates the alternative splicing of VEGF in favour of anti-angiogenic isoforms. Woolard, J.; Vousden, W.; Moss, S. J.; Krishnakumar, A.; Gammons, M. V. R.; Nowak, D. G.; Dixon, N.; Micklefield, J.; Spannhoff, A.; Bedford, M. T.; Gregory, M. A.; Martin, C. J.; Leadlay, P. F.; Zhang, M. Q.; Harper, S. J.; Bates, D. O.; Wilkinson, B.  Chem Sci, 2011, 2, 273-278.  DOI:10.1039/C0SC00297F
  • Protein micro- and nanopatterning using aminosilanes with protein-resistant photolabile protecting groups. Alang Ahmad S, Wong L, Ul-Haq E, Hobbs J, Leggett G, Micklefield J.  J. Am. Chem. Soc., 2011, 133(8), 2749-59.  DOI:10.1021/ja1103662
  • Active site modification of the β-ketoacyl-ACP synthase FabF3 of Streptomyces coelicolor affects the fatty acid chain length of the CDA lipopeptides. Lewis R, Nunns L, Thirlway J, Carroll K, Smith C, Micklefield J.  Chem. Commun. (Camb.), 2011, 47(6), 1860-2. DOI:10.1039/c0cc03444d
  • Biophysical and cellular-uptake properties of mixed-sequence pyrrolidine-amide oligonucleotide mimics. Worthington R, Micklefield J.  Chem. Eur. J. 2011, December; 17(51): 14429-14441. eScholarID:147585 | PMID:22076794 | DOI:10.1002/chem.201101950
  • The Snomipede: A Parallel Platform for Scanning Near-Field Photolithography. ul-Haq E, Liu Z, Wong L, Hobbs J, Leggett G, Micklefield J, Roberts C, Weaver J.  J Mater Res., 2011, 26, 2997-3008.  DOI:10.1557/jmr.2011.370

2010

  • A non-enzymatic, DNA template-directed morpholino primer extension approach. Bell N, Wong R, Micklefield J. Chem. Eur. J.  2010, February; 16(7): 2026-2030. eScholarID:77990 | PMID:20087911 | DOI:10.1002/chem.200902237
  • Reengineering orthogonally selective riboswitches. Dixon N, Duncan J, Geerlings T, Dunstan M, McCarthy J, Leys D, Micklefield J.  Proc. Natl. Acad. Sci. U.S.A. 2010, 107(7), 2830-2835. DOI:10.1073/pnas.0911209107
  • Parallel Scanning Near-Field Photolithography: The Snomipede. Haq E, Liu Z, Zhang Y, Ahmad S, Wong L, Armes S, Hobbs J, Leggett G, Micklefield J, Roberts C, Weaver J. Nano Letters. 2010, October; 10: 4375-4380. eScholarID:100523 | PMID:20945880 | DOI:10.1021/nl1018782
  • Nanoscale biomolecular structures on self-assembled monolayers generated from modular pegylated disulfides.Wong L, Janusz S, Sun S, Leggett G, Micklefield J. Chem. Eur. J.  2010, October; 16(40): 12234-43. eScholarID:100522 | PMID:20839371 | DOI:10.1002/chem.200902439
  • Site-selective immobilisation of functional enzymes on to polystyrene nanoparticles. Wong L, Okrasa K, Micklefield J. Org. Biomol. Chem. 2010, 8(4): 782-787. eScholarID:77988 | PMID:20135034 | DOI:10.1039/b916773k

2009

  • Selective Covalent Protein Immobilization: Strategies and Applications. L. S. Wong, F. Khan, J. Micklefield. Chem. Rev. 2009, 109: 4025-4053. eScholarID:12503 | DOI:10.1021/cr8004668
  • Micrometer- and nanometer-scale photopatterning using 2-nitrophenylpropyloxycarbonyl-protected aminosiloxane monolayers. Ahmad S, Wong L, ul-Haq E, Hobbs J, Leggett G, Micklefield J. J. Am. Chem. Soc. 2009, 131(4): 1513-1522. eScholarID:18292 | PMID:19173668 | DOI:10.1021/ja807612y
  • Chemical Modification of Oligonucleotides for Therapeutic, Bioanalytical and other Applications. Bell N, Micklefield J. Chembiochem : A European Journal of Chemical Biology. 2009, September; 17: 2691-2703. eScholarID:18265 | PMID:19739190 | DOI:10.1002/cbic.200900341
  • Lipase-catalysed kinetic resolutions of secondary alcohols in pressurised liquid hydrofluorocarbons. Corr S, Ball A, Micklefield J. Tetrahedron Lett. 2009, (50): 3543-3546. eScholarID:18269 | PMID:19739190 | DOI:10.1016/j.tetlet.2009.03.037
  • Biosynthesis and biosynthetic engineering of calcium-dependent lipopeptide antibiotics. Micklefield J. Pure Appl. Chem. 2009, 81: 1065-1074. eScholarID:18270 | PMID:19739187 | DOI:10.1351/PAC-CON-08-08-29
  • Structure-Guided Directed Evolution of Alkenyl and Arylmalonate Decarboxylases. Okrasa K, Levy C, Wilding M, Goodall M, Baudendistel N, Hauer B, Leys D, Micklefield J. Angew. Chem. Int. Ed. Engl. 2009, 48: 7691-7694. eScholarID:13316 | PMID:19739187| DOI:10.1002/anie.200904112
  • Biosynthesis of nonribosomal peptide precursors. Wilkinson B, Micklefield J. Meth. Enzymol. 2009, 458: 353-378. eScholarID:18266 | PMID:19374990 | DOI:10.1016/S0076-6879(09)04814-9

2008

  • Auxotrophic-precursor directed biosynthesis of nonribosomal lipopeptides with modified tryptophan residues. B. Amir-Heidari, J. thirlway and J. Micklefield. Org. Biomol. Chem. 2008, 6: 975-978. eScholarID:1a6714 | DOI:10.1039/b718766c
  • Combinatorial biosynthesis of nonribosomal lipopeptides. J. Micklefield. Chemica Oggi. 2008, January; 26: 6-8. eScholarID:1a12240 | DOI:10.1002/chin.200949266
  • Direct site-selective covalent protein immobilization catalyzed by a phosphopantetheinyl transferase. L. S. Wong, J. Thirlway and J. Micklefield. J. Am. Chem. Soc. 2008, 130: 12456-12464. eScholarID:17302 | DOI:10.1021/ja8030278
  • Natural products hit the targets other molecules cannot reach. J. Micklefield. Chemical Biology. 2008, 3: 7-7. eScholarID:1a6731
  • New Malonate Decarboxylases for Industrial Applications K. Okrasa, C. Levy, N. Baudistel, B. Hauer, D. Leys and J. Micklefield. PCT Int. Appl. WO 2009068308 2008, eScholarID:13a38
  • RNA selective cross-pairing of backbone extended pyrrolidine-amide oligonucleotide mimics (bePOMs). R. Worthington, N. Bell and J. Micklefield. Org. Biomol. Chem. 2008, January; 6: 92-103. eScholarID:1a6716 | DOI:10.1039/b714580m
  • Structure and mechanism of an unusual malonate decarboxylase and related racemases. K. Okrasa, C. Levy, N. Baudistel, D. Leys and J. Micklefield. Chem. Eur. J. 2008, 6: 6609-6613. eScholarID:1a12241 | DOI:10.1002/chem.200800918
  • Subsurface biomolecular imaging of Streptomyces coelicolor using mass spectrometry. S. Vaidyanthan, J. S. Fletcher, N. P. Lockyer, R. Goodacre, J. Micklefield and J. C. Vickerman. Anal. Chem. 2008, 80: 1942-1951. eScholarID:1a6715 | DOI:10.1021/ac701921e

2007

  • An asparagine oxygenase (AsnO) and a 3-hydroxyasparaginyl phosphotransferase (HasP) are involved in the biosynthesis of calcium-dependent lipopeptide antibiotics. J. Neary, A. Powell, L. Gordon, C. Milne, F. Flett, B. Wilkinson, C.P. Smith and J. Micklefield. Microbiology. 2007, 153: 768-776. eScholarID:1a771 | DOI:10.1099/mic.0.2006/002725-0
  • Cellular targets of natural products. N. Dixon, L. S. Wong, T. Geerlings and J. Micklefield. Nat. Prod. Rep. 2007, 24: 1288-1310. eScholarID:1a6733 | DOI:10.1039/b616808f
  • Engineered biosynthesis of nonribosomal lipopeptide antibiotics with modified fatty acid side chains. A. Powell, M. Borg B. Amir-Heidari, J. M. Neary, J. Thirlway, B. Wilkinson, C.P. Smith and J. Micklefield. J. Am. Chem. Soc. 2007, 129: 15182-15191. eScholarID:1a6717 | DOI:10.1021/ja074331o
  • Homopolymeric pyrrolidine-amide oligonucleotide mimics: Fmoc-synthesis and DNA/RNA binding properties. S.T.H. Tan, R. Worthington, A. O’Rourke J. Morral R. G. Pritchard and J. Micklefield. Org. Biomol. Chem.  2007, 5: 239-248. eScholarID:1a6722 | DOI:10.1039/b613384n
  • Mining and engineering natural-product biosynthetic pathways. B. Wilkinson and J. Micklefield. Nat. Chem. Biol.. 2007, 3: 379-386. eScholarID:1a6732 | DOI:10.1038/nchembio.2007.7
  • Mixed-sequence pyrrolidine-amide oligonucleotide mimics: Boc(Z) synthesis and DNA/RNA binding properties. R. Worthington, A. O’Rourke J. Morral, S.T.H. Tan and J. Micklefield. Org. Biomol. Chem. 2007, 5: 249-259. eScholarID:1a772 | DOI:10.1039/b613386j
  • NMR confirmation that tryptophan dehydrogenation occurs with syn-stereochemistry during the biosynthesis of CDA in Streptomyces coelicolor. B. A. Heidari and J. Micklefield. J. Org. Chem. 2007, 72: 8950-8953. eScholarID:1a6719 | DOI:10.1021/jo701660v
  • Precursor-directed biosynthesis of nonribosomal lipopeptides with modified glutamate residues. A. Powell, M. Al Nakeeb, B. Wilkinson and J. Micklefield. Chem. Commun. 2007, 2683-2685. eScholarID:1a6720 | DOI:10.1039/b706224a
  • Process for dynamic kinetic resolution (DKR) of racemic compounds in hydrofluorocarbon solvents. J. Micklefield, A. J. Ball and S. Corr. PCT Int. Appl. WO 2007129018 2007, eScholarID:13a39
  • Stereochemical course of tryptophan dehydrogenation during biosynthesis of the calcium-dependent lipopeptide antibiotics. B. Amir-Heidari, J. Thirlway, and Jason Micklefield. Org. Lett. 2007, 9 : 1513-1516. eScholarID:17444 | DOI:10.1021/ol0701619
  • Stereospecific enzymatic transformation of α-ketoglutarate to (2S,3R)-3-methyl glutamate during acidic lipopeptide biosynthesis. C. Mahlert, F.Kopp, J. Thirlway, J. Micklefield, and M. A. Marahiel. J. Am. Chem. Soc. 2007, 129: 12011-12018. eScholarID:1a6718 | DOI:10.1021/ja074427i

2006

  • Biosynthesis of the (2S,3R)-3-Methyl Glutamate Residue of Nonribosomal Lipopeptides. C. Milne, A. Powell, J. Jim, M. Al Nakeeb C. P. Smith and J. Micklefield. J. Am. Chem. Soc. 2006, August; 128: 11250-11259. eScholarID:1a9548 | DOI:10.1021/ja062960c
  • Effects of stereospecific backbone methylation on the DNA/RNA binding properties of pyrrolidine-amide oligonucleotide mimics (cPOM). A. Kahn and J. Micklefield. Chem. Commun. 2006, 1436-1438. eScholarID:1a524 | DOI:10.1039/b518171b

2005

  • Engineering the biosynthesis of nonribosomal lipopeptide antibiotics. C.P. Smith and J. Micklefield. In: M. G. Hicks & C. Kettner, ed. The Chemical Theatre of Biological Systems (Logos Verlag-Berlin). Berlin, Logos Verlag-Berlin.2005, eScholarID:36107
  • Nature’s protection racket. J. Micklefield. Chemistry and Biology. 2005, 12: 1-3. eScholarID:1a6735 | DOI:10.1016/j.chembiol.2005.06.002
  • Reagents for carbonyl methylenation in organic synthesis. Curr. Org. Synth. I. G. Beadham and J. Micklefield. 2005, 2: 231-259. eScholarID:1a6734 | DOI:10.2174/1570179053545396

2004

  • A dynamic combinatorial screen for novel imine reductase activity.  H. Li, P. Williams, J. Micklefield, J. M. Gardiner and G. Stephens. Tetrahedron. 2004, 60: 753-758. eScholarID:1a4487
  • Active-site modifications of adenylation domains lead to hydrolysis of upstream nonribosomal peptidyl thioester intermediates. MICKLEFIELD J; UGURU GABRIEL C.; MILNE CLAIRE; BORG MATTHEW; . J. Am. Chem. Soc. 2004, April; 126(16): 5032-5033. eScholarID:1a9549 | DOI:10.1021/ja048778y
  • Biotransformations in low-boiling hydrofluorocarbon solvents. MICKLEFIELD J; SAUL, SIMON; CORR, STUART. Angew. Chem. 2004, October; 43(41): 5519-5523. eScholarID:1a9550 | DOI:10.1002/anie.200460082
  • Daptomycin Structure and Mechanism of Action Revealed. J. Micklefield. Chemistry & Biology. 2004, 11: 887-888. eScholarID:1a4604
  • Enzymatic process for stereo-selective preparation of chemical compounds in hydrofluorocarbon solvents S.J.P. Saul, J. Micklefield and S. Corr. 2004, eScholarID:13a40
  • Metabolic flux analysis for calcium dependent antibiotic (CDA) production in Streptomyces coelicolor. H. B. Kim, C. P. Smith, J. Micklefield and F. Mavituna. Metab. Eng. 2004, 6: 313-325. eScholarID:1a4603
  • NMR structure determination and calcium binding effects of lipopeptide antibiotic daptomycin. L.-J. Ball, C.M. Goult, J.A. Donarski, J. Micklefield and V. Ramesh. Org. Biomol. Chem. 2004, 2: 1872-1878. eScholarID:1a4485
  • Nucleic acid binding properties of thyminyl and adeninyl pyrrolidine-amide oligonucleotide mimics (POM). Tan, T. H. Samuel; Hickman, David T.; Morral, Jordi; Beadham, Ian G.; Micklefield, Jason. Chem. Commun. 2004, 516-517. eScholarID:1a4486
  • Superior nucleic acid binding properties of thyminyl and adeninyl pyrrolidine-amide oligonucleotide mimics (POM). T.H.S. Tan, D. T. Hickman, J. Morral, I. G. Beadham and J. Micklefield. Chem Commun. 2004, 516-517. eScholarID:1a5088

2003

  • Design, synthesis, conformational analysis and nucleic acid hybridization properties of thymidyl pyrrolidine-amide oligonucleotide mimics (POM). D. T. Hickman, T. H. S. Tan, J. Morral, P. M. King, M. A. Cooper and J. Micklefield. Org. Biomol. Chem. 2003, 1: 3277-3292. eScholarID:1a4488

2002

  • Structure, biosynthetic origin, and engineered biosynthesis of calcium-dependent antibiotics from Streptomyces coelicolor. Z. Hojati, C. Milne, B. Harvey, L. Gordon, M. Borg, F. Flett, B. Wilkinson, P. J. Sidebottom, B. A. M. Rudd, M. A. Hayes, C. P. Smith§ and J. Micklefield. Chemistry & Biology. 2002, 9 (11): 1175-1187. eScholarID:1a212| DOI:10.1016/S1074-5521(02)00252-1
  • Synthesis and nucleic-acid-binding properties of sulfamide- and 3′-N-sulfamate-modified DNA. K. J. Fettes, N. Howard, D. T. Hickman, S. A. Adah, M. R. Player, P. F. Torrence and J. Micklefield. J. Chem. Soc., Perkin Trans. 2002, 1: 485-495. eScholarID:1a3555

2001

  • Backbone modification of nucleic acids: synthesis, structure and therapeutic applications. Micklefield, Jason. Curr. Med. Chem. 2001, 8: 1157-1179. eScholarID:1a3478
  • Kinetically selective binding of single stranded RNA over DNA by a pyrrolidine-amide oligonucleotide mimic (POM). D. T. Hickman, P. M. King, J. M. Slater, M. A. Cooper and J. Micklefield. Nucleosides, Nucleotides & Nucleic Acids. 2001, 20: 1169-1172. eScholarID:1a3477

2000

  • Replacement of the phosphodiester linkage in DNA with sulfamide and 3´-N-sulfamate groups. K. J. Fettes, N. Howard, D. T. Hickman, S. A. Adah, M. R. Player, P. F. Torrence and J. Micklefield. Chem. Commun. 2000, 765-766. eScholarID:1a5820
  • Unusual RNA & DNA binding properties of a novel pyrrolidine-amide oligonucleotide mimics (POM). D. T. Hickman, P. M. King, M. A. Cooper, J. M. Slater and J. Micklefield. Chem. Commun. 2000, 2251-2252. eScholarID:1a5819