Brian Law graduated from the University of Manchester with a BSc. in Cell Biology in 2009. The penultimate year of his degree was spent on placement at AstraZeneca, where he worked on the development of a high-throughput multi-parallel protein purification system. His final year project was spent with Finbarr Hayes where he studied low-copy number plasmid partitioning systems. Brian obtained his PhD with the Micklefield group; his projects centred on O-methyltransferases for biocatalysis and natural product alkyldiversification, and the biosynthetic engineering of nonribosomal peptide antibiotics. Brian has experience in molecular biology, microbiology, protein expression and purification, enzyme assays and kinetics, and analytical techniques including HPLC and mass spectrometry. Brian has continued with the Micklefield group as a postdoc, working as part of a BBSRC sLoLa with Syngenta on natural product biosyntheses.
Publications:
A flavin-dependent decarboxylase-dehydrogenase-monooxygenase assembles the warhead of α, β-epoxyketone proteasome inhibitors. D. Zabala; J. W. Cartwright; D. M. Roberts; B. J. C. Law; L. Song; M. Samborskyy; P. F. Leadlay; J. Micklefield; G. L. Challis J. Am. Chem. Soc. 2016 138: 4342–4345 (http://dx.doi.org/10.1021/jacs.6b01619)
An Enzyme Cascade for Selective Modification of Tyrosine
Residues in Structurally Diverse Peptides and Proteins. A.-W. Struck, M. R. Bennett, S. A. Shepherd, B. J. C. Law, Y. Zhuo, L. S. Wong, J. Micklefield J. Am. Chem. Soc. 2016 138, 3038-3045 (http://dx.doi.org/10.1021/jacs.5b10928)
Effects of Active Site Modification and Quaternary Structure on the Regioselectivity of Catechol-O-Methyltransferase. B. J. C. Law, M. R. Bennett, M. L. Thompson, C. Levy, Sarah A. Shepherd, D. Leys, and J. Micklefield Angew. Chem. Int. Ed. 2016 55: 2683-2687 (http://dx.doi.org/)
Engineered Biosynthesis of Enduracidin Lipoglycopeptide Antibiotics using the Ramoplanin Mannosyltransferase Ram29. M.-C. Wu, M.Q. Styles, B. J. C. Law, A.-W. Struck, L. Nunns, J. Micklefield Microbiology 2015 161: 1338-1347 DOI:10.1099/mic.0.000095
Site-specific Bioalkylation of Rapamycin by the RapM 16-O-Methyltransferase. B. J. C. Law, A.-W. Struck, M. R. Bennett, B. Wilkinson and J. Micklefield Chemical Science 2015 6: 2885-2892 (http://dx.doi.org/10.1039/C5SC00164A)
Bioengineering natural product biosynthetic pathways for therapeutic applications. M.-C. Wu, B. Law, B. Wilkinson and J. Micklefield Current Opinion Biotech. 2012, 23: 931-940 (http://dx.doi.org/10.1016/j.copbio.2012.03.008)
Conference contributions, other (presenting author is underlined)
M. Styles, M.-C. Wu, L. Nunns, B. Law, J. Micklefield, “Combinatorial Biosynthesis of Non-Ribosomal Peptide Antibiotics” 2nd RSC Symposium on Chemical Biology for Drug Discovery, 2012 March 20-21, Alderley Park Conference Centre, Cheshire, UK