Publications:
An asparagine oxygenase (AsnO) and a 3-hydroxyasparaginyl phosphotransferase (HasP) are involved in the biosynthesis of calcium dependent lipopeptide antibiotics. J. Neary, A. Powell, L. Gordon, C. Milne, F. Flett, B. Wilkinson, C.P. Smith and J. Micklefield Microbiology 2007, 153, 768-776. (http://dx.doi.org/10.1099/mic.0.2006/002725-0)
Structure, biosynthetic origin, and engineered biosynthesis of calcium-dependent antibiotics from Streptomyces coelicolor. Z. Hojati, C. Milne, L. Gordon, M. Borg, F. Flett, B. Wilkinson, P. J. Sidebottom, B.A.M. Rudd, M.A. Hayes, C.P. Smith and J. Micklefield Chemistry & Biology 2002, 9, 1175-1187. (http://dx.doi.org/10.1016/s1074-5521(02)00252-1). Featured as the lead article and was illustrated on the fron cover “Chemobiosynthesis leads to new lipopeptide antibiotics”. This paper was also reviewed “New compounds by combining modern genomics and old-fashioned mutasynthesis” by Wohlleben, W.; Pelzer, S. Chemistry & Biology 2002, 9, 1163
Conference contributions, refereed:
C.E. Milne, Z. Hojati, L. Gordon, J. Jim, G. Uguru, C.P. Smith and J. Micklefield “Structure, biosynthetic origin and engineered biosynthesis of Calcium Dependent Antibiotic from Streptomyces Coelicolor” Abstr. Pap. Am. Chem. Soc. 2003, 226, ORGN-534.
Conference contributions, other (presenting author is underlined):
C. Milne, L. Gordon, Z. hojati, J. Jim, G. Uguru, C.P. Smith and J. Micklefield. “Structure, biosynthetic origin and engineered biosynthesis of calcium dependent antibiotics from Streptomyces Coelicolor.” RSC Postgraduate Symposium on Organic and Medicinal Chemistry, Cambridge University, 9 Dec 2002. (oral/abstract).
C. Milne, L. Gordon, Z. hojati, J. Jim, G. Uguru, C.P. Smith and J. Micklefield. “Structure, biosynthetic origin and engineered biosynthesis of calcium dependent antibiotics from Streptomyces Coelicolor.” RSC Bio-organic Symposium, St. Johns College, Cambridge, 27 Nov 2002. (oral/abstract).
L. Gordon, Z. Hojati, C. Milne, B.A.M. Rudd, M. A. Hayes, J. Micklefield and C.P. Smith. “Characterisation of natural and engineered variants of calcium-dependent antibiotics of Streptomyces coelicolor and manipulation of the genes involved in its biosynthesis.” Seventh international Conference on the Biotechnology of Microbial Natural Products (BMP) 2002, Honolulu, Hawaii, Oct 27-30, 2002, pp. 35 (poster).
J. Micklefield, A.E. Hayes, Z. H. Najafabadi, G.C. Uguru, J.M. Neary, C. Milne, L. Gordon, J. Jim, M. Borg, F. Flett and C.P. Smith. “CDA biosynthesis: towards the engineered biosynthesis of novel lipopeptide antibiotics.” BBSRC Workshop on Natural Products Biology, University of Warwick, 21-22 May 2001 (poster).
J. Micklefield, A.E. Hayes, Z. H. ajafabadi, G.C. Uguru, J.M. Neary, C. Milne, L. Gordon, J. Jim, M. Borg, F. Flett and C.P. Smith. “Characterisation of the nonribosomal peptide synthetases and other key enzymes involved in CDA biosynthesis: Towards the engineered biosynthesis of novel lipopeptide antibiotics.” Conference on Streptomyces, University of Warwick, Feb 2001. (Poster).