Jenny gained her PhD at the University of Nottingham. Following on from her time with the Micklefield lab, she moved onto Eden Biodesign Ltd, National Biomanufacturing Centre, Estuary Banks, Liverpool.
Publications:
Introduction of a Non-Natural Amino Acid into a Nonribosomal Peptide Antibiotic by Modification of Adenylation Domain Specificity. J. Thirlway, R. Lewis, L. Nunns, M. Al Nakeeb, M. Styles, A.-W. Struck, C.P. Smith and J. Micklefield Angew. Chem. Int. Ed 2012, 51, 7181-7184 (http://dx.doi.org/10.1002/anie.201202043)
Active site modification of the ẞ-ketoacyl-ACP synthase FabF3 of Streptomyces coelicolor affects the fatty acid chain length of CDA lipopeptides. R. A. Lewis, L. Nunns, J. Thirlway, K. Carroll, C.P. Smith and J Micklefield Chem. Commun. 2011, 47, 1860-1862. (http://dx.doi.org/10.1039/c0cc03444d)
Direct site-selective covalent protein immobilization catalyzed by a phosphopantetheinyl transferase. L.S. Wong, J. Thirlway, and J. Micklefield J. Am. Chem. Soc. 2008, 1430, 12456-12464. (http://dx.doi.org/10.1021/ja8030278)
Auxotrophic-precursor directed biosynthesis of nonribosomal lipopeptides with modified tryptophan residues. B. Amir-Heidari, J. Thirlway, and J. Micklefield Org. Biomol. Chem. 2008, 6, 975-978. Highlighted on the front cover. (http://dx.doi.org/10.1039/b718766c)
Engineered biosynthesis of nonribosomal lipopeptide antibiotics with modified fatty acid side chains. A. Powell, M. Borg, B. Amir-Heidari, J.M. Neary, J. Thirlway, B. Wilkinson, C.P. Smith and J. Micklefield J. Am. Chem. Soc. 2007, 129, 15182-15191. Articles based on this paper were published in the Daily Express, Chemistry & Industry, Manchester Evening News see below. (http://dx.doi.org/10.1021/ja074331o)
Stereospecific enzymatic transformation of a-ketoglutarate to (2S,3R)-3-methyl glutamate during acidic lipopeptide biosynthesis. C. Mahlert, F. Kopp, J. Thirlway, J. Micklefield and M.A. Marahiel J. Am. Chem. Soc 2007, 129, 12011-12018. (http://dx.doi.org/10.1021/ja074427i)
Stereochemical course of tryptophan dehydrogenation during calcium-dependent lipopeptide antibiotic biosynthesis. B.A. Heidari, J. Thirlway and J. Micklefield Org. Lett. 2007, 9, 1513-1516. (http://dx.doi.org/10.1021/ol0701619)
Patents
R. Lewis, C. Smith, J. Thirlway, J. Micklefield, L. Nunns, “Novel calcium dependent antibiotic variants with modified fatty acid side-chains produced by streptomyces strains expressing a mutant ẞ-ketoacyl-ACP synthase II gene” Brit. UK Pat Appl. (2012), GB 2483075 A 20120229
Conference contributions, other (presenting author is underlined)
J. Thirlway, J. Micklefield “Biosynthetic engineering of nonribosomal peptide antibiotics.” The Chemistry and Biology of Natural Product Biosynthesis III. School of Chemistry, University of Bristol, June 20 2007. (oral/abstract)
L.S. Wong, J. Thirlway, J. Micklefield “Site-selective covalent protein immobilization catalysed by a promiscuous phosphopantetheinyl transferase.” Surface Science for Biologically Important Interfaces (SSBII) 9th Annual Meeting, Manchester, 13-14 Sept 2007. (oral/abstract)