Publications:
Engineered Biosynthesis of Enduracidin Lipoglycopeptide Antibiotics using the Ramoplanin Mannosyltransferase Ram29. M.-C. Wu, M.Q. Styles, L. Nunns, B.J.C. Law, J. Micklefield 2015 161: 1338-1347 DOI:10.1099/mic.0.000095
Introduction of a Non-Natural Amino Acid into a Nonribosomal Peptide Antibiotic by Modification of Adenylation Domain Specificity. J. Thirlway, R. Lewis, L. Nunns, M. Al Nakeeb, M. Styles, A.-W. Struck, C.P. Smith and J. Micklefield Angew. Chem. Int. Ed 2012, 51, 7181-7184 (http://dx.doi.org/10.1002/anie.201202043)
Active site modification of the ẞ-ketoacyl-ACP synthase FabF3 of Streptomyces coelicolor affects the fatty acid chain length of CDA lipopeptides. R. A. Lewis, L. Nunns, J. Thirlway, K. Carroll, C.P. Smith and J Micklefield Chem. Commun. 2011, 47, 1860-1862. (http://dx.doi.org/10.1039/c0cc03444d)
Patents
R. Lewis, C. Smith, J. Thirlway, J. Micklefield, L. Nunns, “Novel calcium dependent antibiotic variants with modified fatty acid side-chains produced by streptomyces strains expressing a mutant ẞ-ketoacyl-ACP synthase II gene” Brit. UK Pat Appl. (2012), GB 2483075 A 20120229
Conference contributions, other (presenting author is underlined)
M. Styles, M.-C. Wu, L. Nunns, B. Law, J. Micklefield, “Combinatorial Biosynthesis of Non-Ribosomal Peptide Antibiotics” 2nd RSC Symposium on Chemical Biology for Drug Discovery, 2012 March 20-21, Alderley Park Conference Centre, Cheshire, UK