Sarah Shepherd studied for a MChem degree in Chemistry with Medicinal Chemistry at the University of Warwick from 2006-2010. During her fourth year MChem project, her research involved the synthesis of inhibitors of carotenoid cleavage dioxygenases, under the supervision of Professor Tim Bugg.
Sarah joined the Micklefield group in 2010 and has achieved a PhD in the area of biocatalysis. Sarah is researching halogenase enzymes that chlorinate and brominate aromatic substrates. Sarah has experience in cloning and mutagenesis, protein purification and expression and analytical techniques such as, HPLC, mass spectrometry and NMR. Currently working as Network Manager for NPRONET (Natural products discovery and bioengineering network).
Outside of the lab, Sarah enjoys hockey and golf.
Publications:
- Structure and Biocatalytic Scope of Coclaurine N-Methyltransferase. M. R. Bennett, M. L. Thompson, S. A. Shepherd, M. S. Dunstan, A. J. Herbert, D. R. M. Smith, V. A. Cronin, B. R. K. Menon, C. Levy & J. Micklefield Angew. Chem. Int. Ed. 2018 in press. (http://dx.doi.org/10.1002/anie.201805060)
- Development of Halogenase Enzymes for Use in Synthesis J. Latham, E. Brandenburger, S. A. Shepherd, B. R. K. Menon and J.Micklefield Chem. Rev 2018, 118, 232-269; (http://dx.doi.org/10.1021/acs.chemrev.7b00032)
- RadH: A Versatile Halogenase for Integration into Synthetic Pathways. B. R. K. Menon, E. Brandenburger, H. H. Sharif, U. Klemstein, S.A. Shepherd, M. F. Greaney, and J. Micklefield Angew. Chem. Int. Ed. 2017 in press (http://dx.doi.org/10.1002/anie.201706342)
- Development of Halogenase Enzymes for use in Synthesis. Jonathan Latham, Eileen Brandenburger, Sarah A. Shepherd, Binuraj R. K. Menon and Jason Micklefield. American Chemical Society. 2017, (DOI: 10.1021/acs.chemrev.7b00032)
- Recent Advances in Methyltransferase Biocatalysis. M.R. Bennett, S.A. Shepherd, V.A. Cronin, J. Micklefield Current Opinion in Chemical Biology. 2017, in press.
- Structure and Biocatalytic Scope of Thermophillic Flavin-Dependent Halogenase and Flavin Reductase Enzymes B. R. K. Menon, J. Latham, M. S. Dunstan, E. Brandenburger, U. Klemstein, S. A. Shepherd, C. Karthikeyan, M. F. Greaney, D. Leys and J. Micklefield* Org. Biomol. Chem. 2016, 14, 9354–9361. (http://dx.doi.org/10.1039/C6OB01861K)
- Integrated Catalysis Opens New Arylation Pathways via Regiodivergent Enzymatic C-H Activation. J. Latham, J.-M. Henry, H. H. Sharif, B. R. K. Menon, S. A. Shepherd, M. F. Greaney, J. Micklefield. Nature Commun. 2016, 7, 11873 (http://dx.doi.org/10.1038/NCOMMS11873)
- An Enzyme Cascade for Selective Modification of Tyrosine
Residues in Structurally Diverse Peptides and Proteins. A.-W. Struck, M. R. Bennett, S. A. Shepherd, B. J. C. Law, Y. Zhuo, L. S. Wong, J. Micklefield J. Am. Chem. Soc. 2016, 138, 3038-3045. http://dx.doi.org/10.1021/jacs.5b10928) - A Structure-Guided Switch in the Regioselectivity of a Tryptophan Halogenase. S. A. Shepherd, B. R. K. Menon, H. Fisk, A.-W. Struck, C. Levy, D. Leys, J. Micklefield ChemBioChem 2016, 17, 9, p. 821–824 (http://dx.doi.org/10.1002/cbic.201600051)
- Effects of Active Site Modification and Quaternary Structure on the Regioselectivity of Catechol-O-Methyltransferase. B. J. C. Law, M. R. Bennett, M. L. Thompson, C. Levy, S. A. Shepherd, D. Leys, and J. Micklefield Angew. Chem. Int. Ed. 2016, 55, 2683-2687. (http://dx.doi.org/10.1002/anie.201508287)
- Biochemical characterization and selective inhibition of b-carotene cis-trans isomerase D27 and carotenoid cleavage dioxygenase CCD8 on the strigolactone biosynthetic pathway. Peter J. Harrison, Sophie A. Newgas, Flora Descombes, Sarah A. Shepherd, Andrew J. Thompson and Timothy D. H. Bugg FEBS Journal., 2015, in press. (DOI: 10.1111/febs.13400)
- Extending the Biocatalytic Scope of Regiocomplementary Flavin-Dependent Halogenase Enzymes. S.A. Shepherd, C. Karthikeyan, J. Latham, A.-W. Struck, M. L. Thompson, B. Menon, C. Levy, D. Leys and J. Micklefield Chemical Science 2015, 6, 3454-3460 (http://dx.doi.org/10.1039/C5SC00913H)
- A High-Throughput Assay for Arylamine Halogenation Based on a Peroxidase Mediated Quinone-Amine Coupling with Applications in the Screening of Enzymatic Halogenations. J. Hosford, S.A. Shepherd, J. Micklefield, L. S. Wong Chem. Eur. J., 2014, 20, 16759-16763 (http://dx.doi.org/10.1002/chem.201403953)