{"id":85,"date":"2012-08-30T09:21:02","date_gmt":"2012-08-30T08:21:02","guid":{"rendered":"http:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/?page_id=85"},"modified":"2018-09-22T09:21:13","modified_gmt":"2018-09-22T08:21:13","slug":"brian-law","status":"publish","type":"page","link":"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/?page_id=85","title":{"rendered":"Dr. Brian Law"},"content":{"rendered":"

Brian Law<\/strong> graduated from the University of Manchester with a BSc. in Cell Biology in 2009. The penultimate year of his degree was spent on placement at AstraZeneca, where he worked on the development of a high-throughput multi-parallel protein purification system. His final year project was spent with Finbarr Hayes where he studied low-copy number plasmid partitioning systems. Brian\u00a0obtained\u00a0his PhD with the Micklefield group; his projects\u00a0centred on\u00a0O<\/em>-methyltransferases for\u00a0<\/span>biocatalysis and natural product alkyldiversification, and the biosynthetic engineering of nonribosomal peptide antibiotics. Brian has experience in molecular biology, microbiology, protein expression and purification, enzyme assays and kinetics, and analytical techniques including HPLC and mass spectrometry. Brian has continued with the Micklefield group as a postdoc, working\u00a0as part of\u00a0a BBSRC sLoLa with Syngenta on natural product biosyntheses.<\/p>\n

Publications:<\/span><\/p>\n

A flavin-dependent decarboxylase-dehydrogenase-monooxygenase assembles the warhead of \u03b1, \u03b2-epoxyketone proteasome inhibitors.<\/strong> D.\u00a0Zabala; J. W. Cartwright; D. M. Roberts; B. J. C. Law<\/strong>; L. Song; M. Samborskyy; P. F. Leadlay; J. Micklefield; G. L. Challis J. Am. Chem. Soc.<\/em> 2016<\/strong>\u00a0138:\u00a04342\u20134345 (http:\/\/dx.doi.org\/<\/span>10.1021\/jacs.6b01619<\/a>)<\/em><\/p>\n

An Enzyme Cascade for Selective Modification of Tyrosine
\nResidues in Structurally Diverse Peptides and Proteins.\u00a0<\/strong>A.-W. Struck, M. R. Bennett, S. A. Shepherd, B. J. C. Law<\/strong>, Y. Zhuo, L. S. Wong, J. Micklefield\u00a0J. Am. Chem. Soc.<\/em>\u00a02016 <\/strong>138, 3038-3045\u00a0(http:\/\/dx.doi.org\/10.1021\/jacs.5b10928<\/a>)\u00a0<\/em><\/p>\n

Effects of Active Site Modification and Quaternary Structure on the Regioselectivity of Catechol-O<\/em>-Methyltransferase. <\/strong>B. J. C. Law<\/strong>, M. R. Bennett, M. L. Thompson, C. Levy, Sarah A. Shepherd, D. Leys, and J. Micklefield Angew. Chem. Int. Ed. <\/em>2016 <\/strong>55: 2683-2687<\/em>\u00a0(http:\/\/dx.doi.org\/10.1002\/anie.201508287<\/span><\/span><\/a>)<\/p>\n

Engineered Biosynthesis of Enduracidin Lipoglycopeptide Antibiotics using the Ramoplanin Mannosyltransferase Ram29.\u00a0<\/strong>M.-C. Wu,\u00a0<\/strong>M.Q. Styles, B. J. C. Law<\/strong>, A.-W. Struck, L. Nunns, J. Micklefield\u00a0Microbiology<\/em> 2015<\/strong> 161: 1338-1347<\/em>\u00a0<\/em>DOI:10.1099\/mic.0.000095<\/a><\/p>\n

Site-specific Bioalkylation of Rapamycin by the RapM 16-O<\/em>-Methyltransferase.\u00a0B. J. C. Law<\/strong>,\u00a0A.-W. Struck, M. R. Bennett, B. Wilkinson and J. Micklefield\u00a0Chemical Science<\/em>\u00a02015<\/strong>\u00a06:\u00a0<\/em><\/em>2885-2892\u00a0(http:\/\/dx.doi.org\/10.1039\/C5SC00164A<\/span><\/a>)\u00a0\u00a0<\/em><\/p>\n

Bioengineering natural product biosynthetic pathways for therapeutic applications.\u00a0<\/strong>M.-C. Wu, B. Law<\/strong>, B. Wilkinson and J. Micklefield\u00a0Current Opinion Biotech.<\/em>\u00a02012<\/strong>, 23: 931-940 (http:\/\/dx.doi.org\/10.1016\/j.copbio.2012.03.008<\/a>)<\/p>\n

Conference contributions, other (presenting author is underlined)\u00a0<\/span><\/p>\n

M. Styles<\/span>,\u00a0M.-C. Wu, L. Nunns, B. Law<\/strong>, J. Micklefield, “Combinatorial Biosynthesis of Non-Ribosomal Peptide Antibiotics” 2nd RSC Symposium on Chemical Biology for Drug Discovery, 2012 March 20-21, Alderley Park Conference Centre, Cheshire, UK<\/p>\n","protected":false},"excerpt":{"rendered":"

Brian Law graduated from the University of Manchester with a BSc. in Cell Biology in 2009. The penultimate year of his degree was spent on placement at AstraZeneca, where he worked on the development of a high-throughput multi-parallel protein purification … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-85","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/index.php?rest_route=\/wp\/v2\/pages\/85","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=85"}],"version-history":[{"count":28,"href":"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/index.php?rest_route=\/wp\/v2\/pages\/85\/revisions"}],"predecessor-version":[{"id":1061,"href":"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/index.php?rest_route=\/wp\/v2\/pages\/85\/revisions\/1061"}],"wp:attachment":[{"href":"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=85"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}