{"id":115,"date":"2012-09-02T18:47:51","date_gmt":"2012-09-02T17:47:51","guid":{"rendered":"http:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/?page_id=115"},"modified":"2016-04-25T15:39:59","modified_gmt":"2016-04-25T14:39:59","slug":"dr-ming-cheng-wu","status":"publish","type":"page","link":"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/?page_id=115","title":{"rendered":"Dr. Ming-Cheng Wu"},"content":{"rendered":"

Ming-Cheng completed his Master degree in Insect Physiology and Biochemistry from the National Chung-Hsing University, Taiwan in 2004. After graduation, he stayed in academia as a research assistant carrying out two subjects: (1) development of biochip for discrimination of Flaviviruses and major lepidopterous pests of Rosaceae fruits; (2) Expression and purification of anti-bacterial peptide cecropin from yeast, Saccharomyces cerevisiae, and transgenic <\/em>tomato. In 2008, he joined in the Micklefield group as a PhD student; his projects were involved in engineering biosynthesis of chlorination and mannosylation on non-ribosomal peptide antibiotics\u00a0enduracidin and ramoplanin, and re-engineering of a preQ1<\/sub> riboswitch as a tool for regulating gene expression. He obtained his PhD degree in 2011 and remains in the Micklefield group working on a Post-doctoral position continuing his works on engineering biosynthesis and riboswitches. Ming-Cheng has experience in molecular biology, microbiology, and analytical techniques including HPLC and mass spectrometry.<\/p>\n

Publications:<\/span><\/p>\n

Rewiring Riboswitches to Create New Genetic Circuits in Bacteria.\u00a0<\/strong>C.J. Robinson, D. Medina-Stacey, M.-C. Wu<\/strong>, H.A. Vincent, J. Micklefield\u00a0Methods in Enzymology\u00a0<\/em>2016\u00a0<\/strong>in press. (http:\/\/dx.doi.org\/10.1016\/bs.mie.2016.02.022<\/a>)<\/em><\/p>\n

Rational Re-engineering of the Transcriptional Silencing PreQ1 Riboswitch.\u00a0<\/strong>M.-C. Wu<\/strong>, P.T. Lowe, C.J. Robinson, H.A. Vincent, N. Dixon, J. Leigh, J. Micklefield\u00a0J. Am. Chem. Soc.<\/em> 2015<\/strong> 137,\u00a0<\/em>9015-9021\u00a0(http:\/\/dx.doi.org\/10.1021\/jacs.5b03405<\/a>)\u00a0<\/em><\/p>\n

Engineered Biosynthesis of Enduracidin Lipoglycopeptide Antibiotics using the Ramoplanin Mannosyltransferase Ram29. M.-C. Wu,\u00a0<\/strong>M.Q. Styles, L. Nunns, B.J.C. Law, J. Micklefield\u00a02015<\/strong> 161: 1338-1347\u00a0<\/em>DOI:10.1099\/mic.0.000095<\/a><\/p>\n

Modular Riboswitch Toolsets for Synthetic Genetic Control in Diverse Bacterial Species.\u00a0<\/strong>C.J. Robinson, H.A. Vincent,\u00a0M.-C. Wu<\/strong>, P.T.Lowe, M.S. Duncan, D. Leys, and J. Micklefield\u00a0J. Am. Chem. Soc.\u00a0<\/em>2014<\/strong>, 136, 10615-10624. DOI:\u00a010.1021\/ja502873j\u00a0<\/a><\/p>\n

Bioengineering natural product biosynthetic pathways for therapeutic applications. M.-C. Wu<\/strong>, B. Law, B. Wilkinson and J. Micklefield\u00a0Current Opinion Biotech.<\/em>\u00a02012<\/strong>, 23, 931-940 (http:\/\/dx.doi.org\/10.1016\/j.copbio.2012.03.008<\/a>)<\/p>\n

Oligo-nucleotide probes of lepidopteran pests identification, biochip, and identifying method thereof.\u00a0<\/strong>K.H. Lu, Y.H. Chen,\u00a0M.-C. Wu 2009,\u00a0<\/strong>Taiwan Patent 096145246.<\/p>\n

Juvenile hormone induction of glutathione\u00a0S<\/em>-transferase activity in the larval fat body of the common cutworm,\u00a0Spodoptera litura<\/em>\u00a0(Lepidoptera: Noctuidae).\u00a0M.-C. Wu<\/strong>, K.H. Lu\u00a02008,\u00a0<\/strong>Archives of Insect Biochemistry and Physiology\u00a068: 232-240<\/p>\n

Specific primers, oligo-nucleotide probes, biochip of dengue virus identification and identifying method thereof.<\/strong> W.C. Tu, K.H. Lu, M.-C. Wu 2007,\u00a0<\/strong>Taiwan Patent 96150837.<\/p>\n

Conference contributions, other (presenting author is underlined)<\/span><\/p>\n

C.J. Robinson<\/span>, N. Dixon, J.N. Duncan, T. Geerlings, M.-C. Wu<\/strong>,\u00a0P. Lowe, J. Micklefields, “Orthogonal Riboswitches As Tools for Controlling Gene Expression in Bacteria,” RNA 2012: The 17th Annual Meeting of the RNA Society, The University of Michigan, Ann Arbor, Mi, USA. May 29-Jun 2, 2012<\/p>\n

M. Styles<\/span>, M.-C. Wu<\/strong>, L. Nunns, B. Law, J. Micklefield, “Combinatorial Biosynthesis of Non-Ribosomal Peptide Antibiotics” 2nd RSC Symposium on Chemical Biology for Drug Discovery, 2012 March 20-21, Alderley Park Conference Centre, Cheshire, UK<\/p>\n

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Ming-Cheng completed his Master degree in Insect Physiology and Biochemistry from the National Chung-Hsing University, Taiwan in 2004. After graduation, he stayed in academia as a research assistant carrying out two subjects: (1) development of biochip for discrimination of Flaviviruses … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-115","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/index.php?rest_route=\/wp\/v2\/pages\/115","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=115"}],"version-history":[{"count":11,"href":"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/index.php?rest_route=\/wp\/v2\/pages\/115\/revisions"}],"predecessor-version":[{"id":778,"href":"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/index.php?rest_route=\/wp\/v2\/pages\/115\/revisions\/778"}],"wp:attachment":[{"href":"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=115"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}