{"id":11,"date":"2012-08-17T09:57:08","date_gmt":"2012-08-17T08:57:08","guid":{"rendered":"http:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/?page_id=11"},"modified":"2025-06-03T16:28:49","modified_gmt":"2025-06-03T15:28:49","slug":"publications","status":"publish","type":"page","link":"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/?page_id=11","title":{"rendered":"Publications"},"content":{"rendered":"<p>2025:<\/p>\n<ul>\n<li>\n<div><b><span lang=\"EN\">Enzymatic Cascades for Stereoselective and Regioselective Amide Bond Assembly.\u00a0<\/span><\/b><span lang=\"EN\"><span lang=\"EN\"><span lang=\"EN\"><span lang=\"EN\">D. Torri, L. Bering, L. R. L. Yates, S.M. Angiolini, G. Xu, S. Cuesta-Hoyos, S. A. Shepherd &amp; J. Micklefield* <\/span><\/span><\/span><\/span><i class=\"\">Angew. Chem. Int. Ed. <\/i><b class=\"\">2025<\/b>, <i class=\"\">64<\/i>, e202422185 (<a href=\"https:\/\/doi.org\/10.1002\/anie.202422185\">https:\/\/doi.org\/10.1002\/anie.202422185)<\/a><\/div>\n<\/li>\n<li><b>Integrating Enzymes with Photoredox Catalysis for Conversion of Nitriles into Fluorinated Products<\/b><b><span lang=\"EN-US\">. <\/span><\/b><span lang=\"EN-US\">S. Angiolini, I.Bruton, L. Bering, S. Sowerby Thomas, J. Thompson, S. A Shepherd, J. Micklefield. <\/span><i>ChemCatChem <b>2025<\/b><\/i><span lang=\"EN-US\"><span lang=\"EN-US\">, <\/span><\/span>e202500240<span lang=\"EN-US\">\u00a0(<a href=\"https:\/\/doi.org\/10.1002\/cctc.202500240\">https:\/\/doi.org\/10.1002\/cctc.202500240<\/a>)<\/span><\/li>\n<li>\n<div><b><span lang=\"EN-US\">Biocatalytic Synthesis of N-trans-feruloyltyramine Using an Amide Bond Synthetase with an ATP. <\/span><\/b><span lang=\"EN-US\">B. Zhao, J. Micklefield, Y. Wang, F. Wang. <\/span><i><span lang=\"EN-US\">Appl Biochem Biotechnol <\/span><\/i><b><span lang=\"EN-US\">2025, <\/span><\/b><i><span lang=\"EN-US\">in press<\/span><\/i><span lang=\"EN-US\">. (<\/span><span lang=\"EN-US\"><a href=\"https:\/\/doi.org\/10.1007\/s12010-025-05250-z\">https:\/\/doi.org\/10.1007\/s12010-025-05250-z<\/a>)<\/span><\/div>\n<\/li>\n<\/ul>\n<p>2024:<\/p>\n<ul>\n<li><strong>Cryptic enzymatic assembly of peptides armed with \u03b2-lactone warheads. <\/strong>G. Xu, D. Torri, S. Cuesta-Hoyos, D. Panda, L. R. L. Yates, R. Zallot, K. Bian, D. Jia, A. I. Iorgu, C. Levy, S. A. Shepherd &amp; Jason Micklefield* <em>Nat Chem Biol <\/em><strong>2024<\/strong><em>.<\/em> (<a href=\"https:\/\/doi.org\/10.1038\/s41589-024-01657-7\">https:\/\/doi.org\/10.1038\/s41589-024-01657-7<\/a>). Also see research briefing (<a href=\"https:\/\/doi.org\/10.1038\/s41589-024-01658-6\">https:\/\/doi.org\/10.1038\/s41589-024-01658-6<\/a>) &amp; behind the paper blog (<a href=\"https:\/\/go.nature.com\/3LrIXUx\">https:\/\/go.nature.com\/3LrIXUx<\/a>).<\/li>\n<li><b><span lang=\"EN-US\">Searching for the optimal microbial factory: high-throughput biosensors and analytical techniques for screening small molecules.\u00a0 <\/span><\/b><span lang=\"EN-US\">E. O&#8217;Connor, J. Micklefield, Y.Cai <em>Current Opinion in Biotechnology<\/em>, <strong>2024<\/strong>, 87, 103125 (<a href=\"https:\/\/doi.org\/10.1016\/j.copbio.2024.103125\">https:\/\/doi.org\/10.1016\/j.copbio.2024.103125<\/a>)<\/span><\/li>\n<\/ul>\n<p>2023:<\/p>\n<ul>\n<li><b><span lang=\"EN-US\">Biocatalytic approaches to amide synthesis.\u00a0<\/span><\/b><span lang=\"EN-US\">M. Rowlinson &amp; J. Micklefield*\u00a0<em>Comprehensive Chirality<\/em>, 2nd edition,\u00a0<strong>2023<\/strong>, (<a href=\"https:\/\/doi.org\/10.1016\/B978-0-32-390644-9.00083-4\">https:\/\/doi.org\/10.1016\/B978-0-32-390644-9.00083-4<\/a>)<\/span><\/li>\n<\/ul>\n<p>2022:<\/p>\n<ul>\n<li><b><span lang=\"EN-US\">Merging Enzymes with Chemocatalysis for Sustainable Amide Bond Synthesis.\u00a0<\/span><\/b><span lang=\"EN-US\">L. Bering, E. J. Craven, S. A. Sowerby Thomas, S. A. Shepherd &amp; J. Micklefield* <\/span><i>Nature Commun.\u00a0<\/i><b>2022<\/b><b class=\"\"><b class=\"\">,\u00a0<\/b><\/b><i>13,\u00a0<\/i>380.\u00a0(<a href=\"https:\/\/doi.org\/10.1038\/s41467-022-28005-4\">https:\/\/doi.org\/10.1038\/s41467-022-28005-4<\/a>). Also see behind the paper (<a href=\"https:\/\/go.nature.com\/3FCmSxf\">https:\/\/go.nature.com\/3FCmSxf<\/a>) &amp; Chemical &amp; Engineering News (C&amp;EN), &#8220;<a href=\"https:\/\/cen.acs.org\/synthesis\/Enzyme-copper-combo-builds-amide\/100\/i4\">Enzyme and copper combo builds amide bonds<\/a>.&#8221;<\/li>\n<li><strong>New reaction pathways by integrating chemo- and biocatalysis<\/strong>. L. Bering*, J. Thompson &amp; J. Micklefield* <em>Trends in Chemistry<\/em>, <strong>2022<\/strong>, 4, 392-408. (<a href=\"https:\/\/doi.org\/10.1016\/j.trechm.2022.02.008\">https:\/\/doi.org\/10.1016\/j.trechm.2022.02.008<\/a>)<\/li>\n<\/ul>\n<p>2021:<\/p>\n<ul>\n<li><b class=\"\">Discovery, Characterisation and Engineering of Ligases for Amide Synthesis.<\/b>\u00a0 M. Winn, F. Wang, M. Rowlinson, L. Bering, D. Francis, C. Levy &amp;<br \/>\nJ Micklefield\u00a0<span style=\"border: none; font-style: italic;\">Nature<\/span>\u00a0<strong>2021<\/strong>,\u00a0<span style=\"border: none; font-style: italic;\">593<\/span>, 391\u2013398. (<a href=\"https:\/\/doi.org\/10.1038\/s41586-021-03447-w\">https:\/\/doi.org\/10.1038\/s41586-021-03447-w<\/a>); Shareable link (<a href=\"https:\/\/rdcu.be\/cmdd4\">https:\/\/rdcu.be\/cmdd4<\/a>); Also see behind the paper (<a href=\"https:\/\/go.nature.com\/3uQTAqp\">https:\/\/go.nature.com\/3uQTAqp<\/a>).<\/li>\n<li><b class=\"\"><b class=\"\"><span class=\"\"><a href=\"https:\/\/doi.org\/10.1038\/s41467-021-27139-1\"><b>Gene editing enables rapid engineering of complex antibiotic assembly lines<\/b><\/a>. <\/span><\/b><\/b><span class=\"\">W. L. Thong, Y. Zhang, Y. Zhuo, K. J. Robins, J. K. Fyans, A. J. Herbert, B. J. C. Law &amp; J. Micklefield <em>Nature Commun<\/em> <strong>2021<\/strong>, <em>12<\/em>, 6872<\/span><span class=\"\">. Shareable link (<a href=\"https:\/\/rdcu.be\/cmdd4\">https:\/\/<\/a><a class=\"css-4rbku5 css-18t94o4 css-901oao css-16my406 r-1cvl2hr r-1loqt21 r-poiln3 r-bcqeeo r-qvutc0\" dir=\"ltr\" role=\"link\" href=\"https:\/\/t.co\/j9gVycaltZ?amp=1\" target=\"_blank\" rel=\"noopener noreferrer\">rdcu.be\/cB1FR<\/a>); (<a href=\"https:\/\/doi.org\/10.1038\/s41467-021-27139-1\">https:\/\/doi.org\/10.1038\/s41467-021-27139-1<\/a>);\u00a0<\/span><span class=\"\">Behind the paper (<a href=\"https:\/\/go.nature.com\/3nSd9gV\">https:\/\/go.nature.com\/3uQTAqp<\/a>).<\/span><\/li>\n<li><strong>Programmable late-stage C\u2212H bond functionalization enabled by integration of enzymes with chemocatalysis<\/strong>. E. J. Craven, J. Latham, S. A. Shepherd, I. Khan, A. Diaz-Rodriguez, M. F. Greaney &amp; J. Micklefield <em>Nature Catalysis<\/em> <strong>2021<\/strong>, <em>4<\/em>, 385\u2013394. (<a href=\"https:\/\/doi.org\/10.1038\/s41929-021-00603-3\">https:\/\/doi.org\/10.1038\/s41929-021-00603-3<\/a>); Shareable link (<a href=\"https:\/\/rdcu.be\/cmdf6\">https:\/\/rdcu.be\/cmdf6<\/a>); Also see behind the paper (<a href=\"https:\/\/go.nature.com\/3apWdqC\">https:\/\/go.nature.com\/3apWdqC<\/a>).<\/li>\n<li><strong>Genome editing reveals that pSCL4 is required for chromosome linearity in <em>Streptomyces clavuligerus<\/em>.<\/strong> J. P. Gomez-Escribano1, L. A. Gallardo, K. A. J. Bozh\u00fcy\u00fck, S. G. Kendrew, B. D. Huckle, N. A. Crowhurst, M. J. Bibb, A. J. Collis, J. Micklefield, P. R. Herron &amp; Barrie Wilkinson <em>Microbial Genomics<\/em><strong> 2021<\/strong>, <em>7<\/em>, 000669 (<a href=\"https:\/\/doi.org\/10.1099\/mgen.0.000669\">https:\/\/doi.org\/10.1099\/mgen.0.000669<\/a>)<\/li>\n<\/ul>\n<p>2020:<\/p>\n<ul>\n<li><b class=\"\">Engineering orthogonal methyltransferases to create alternative bioalkylation pathways.<\/b> A. \u00a0J. Herbert, S. A. Shepherd, V. A. Cronin, M. R. Bennett, R. Sung &amp; J Micklefield* <i class=\"\">Angew. Chem. Int. Ed. <\/i><b class=\"\">2020<\/b>, <i class=\"\">59<\/i>, 2\u20139. (<a class=\"\" href=\"https:\/\/doi.org\/10.1002\/anie.202004963\">https:\/\/doi.org\/10.1002\/anie.202004963<\/a>); Also see <em>Nature Chemistry<\/em> News &amp; Views (<a href=\"https:\/\/rdcu.be\/cmde1\">https:\/\/rdcu.be\/cmde1<\/a>).<\/li>\n<li><strong>Engineering Escherichia coli towards de novo production of gatekeeper (2S)-flavanones: naringenin, pinocembrin, eriodictyol and homoeriodictyol.<\/strong> Dunstan, M., Robinson, C., Jervis, A., Yan, C., Carbonell, P., Hollywood, K., Currin, A., Swainston, N., Le Feuvre, R., Micklefield, J., Faulon, J-L., Breitling, R., Turner, N., Takano, E. &amp; Scrutton, N. <em>Synthetic Biology<\/em> <strong>2020<\/strong>, in press.(<a href=\"https:\/\/doi.org\/10.1093\/synbio\/ysaa012\">https:\/\/doi.org\/10.1093\/synbio\/ysaa012<\/a>)<\/li>\n<li><b class=\"\">Rapid prototyping of biosynthetic monomers production for enhanced biomanufacturing of materials<\/b>. C. J. Robinson, P. Carbonell, A. J. Jervis, C. Yan, K. A. Hollywood, M. S. Dunstan, A. Currin, R. Spiess, N. Swainston, S. Taylor, P. Mulherin, N. Matthews, K. Malone, R. Le Feuvre, P. Shapira, P. Barran, N. J. Turner, J. Micklefield, R. Breitling, E. Takano, N. S. Scrutton <i class=\"\">Metabolic Engineering <\/i><b class=\"\">2020<\/b>,<b class=\"\"> <\/b><i class=\"\">60<\/i>, 168\u2013182. (<a class=\"\" href=\"https:\/\/doi.org\/10.1016\/j.ymben.2020.04.008\">https:\/\/doi.org\/10.1016\/j.ymben.2020.04.008<\/a>).<\/li>\n<li><strong>Editorial overview: Biocatalysis and biotransformations. <\/strong>D. J. Campopiano &amp; J. \u00a0Micklefield<em> Current Opinion in Chemical Biology<\/em>,\u00a0 2020, 55, A1-A3 (<a href=\"https:\/\/doi.org\/10.1016\/j.cbpa.2020.04.019\">https:\/\/doi.org\/10.1016\/j.cbpa.2020.04.019<\/a>)<\/li>\n<li><span class=\"\"><b class=\"\">Harnessing and engineering amide bond forming ligases for the synthesis of amides.<\/b> M. Winn, S. Richardson, D. J. Campopiano &amp; J. \u00a0Micklefield* <i class=\"\">Current Opinion in Chemical Biology<\/i> <b class=\"\">2020<\/b>, <i class=\"\">55<\/i>, 77\u201385 (<\/span><a class=\"\" href=\"https:\/\/doi.org\/10.1016\/j.cbpa.2019.12.004\"><span class=\"\">https:\/\/doi.org\/10.1016\/j.cbpa.2019.12.004<\/span><\/a><span class=\"\">)<\/span><\/li>\n<\/ul>\n<p>2019:<\/p>\n<ul>\n<li><strong>Engineering Enzymatic Assembly Lines to Produce New Antibiotics<\/strong>. K. A. J. Bozh\u00fcy\u00fck, J. Micklefield, B. Wilkinson\u00a0<em>Current Opinion in Microbiology<\/em>\u00a0<strong>2019<\/strong>,\u00a0<em>51<\/em>, 88-96. (<a href=\"https:\/\/doi.org\/10.1016\/j.mib.2019.10.007\">https:\/\/doi.org\/10.1016\/j.mib.2019.10.007<\/a>)<\/li>\n<li><strong>Streamlined recycling of <em>S<\/em>-adenosylmethione <\/strong>(News &amp; Views). J. Micklefield <em>Nature Catalysis<\/em>\u00a0<strong>2019<\/strong>,<em> 2<\/em>, 644-645. (<a href=\"https:\/\/doi.org\/10.1038\/s41929-019-0323-6\">https:\/\/doi.org\/10.1038\/s41929-019-0323-6<\/a>)<\/li>\n<li><strong>Assembling a plug-and-play production line for combinatorial biosynthesis of aromatic polyketides in <em>Escherichia coli<\/em><\/strong><em>, <\/em>M. Cummings, A. Peters, G. Whitehead, B. K. Menon, J. Micklefield, S. Webb &amp; E. Takano, <em>PLOS Biology <\/em><strong>2019<\/strong>, <em>17<\/em>, e3000347 (<a href=\"https:\/\/doi.org\/10.1371\/journal.pbio.3000347\">https:\/\/doi.org\/10.1371\/journal.pbio.3000347<\/a>)<\/li>\n<\/ul>\n<p>2018<\/p>\n<ul>\n<li><strong>A\u00a0vitamin K-dependent carboxylase is involved in antibiotic biosynthesis<\/strong>.\u00a0B. J. C. Law, Y. Zhuo,\u00a0D. Francis, M. Winn, Y. Zhang, M.\u00a0Samborskyy, A. Murphy, L. Ren, P. F. Leadlay\u00a0&amp; J. Micklefield.\u00a0<em>Nature Catalysis<\/em>\u00a0<strong>2018,<\/strong>\u00a0<em>1<\/em>,\u00a0977\u2013984 (<a href=\"http:\/\/rdcu.be\/bbZ99\">rdcu.be\/bbZ99<\/a>\u00a0). Also see\u00a0<em>Nature Catalysis<\/em>\u00a0News &amp; Views (<a href=\"https:\/\/rdcu.be\/bdd6j\">https:\/\/rdcu.be\/bdd6j<\/a>)\u00a0and Nature Research Community News\u00a0(<a href=\"https:\/\/go.nature.com\/2r4Smbu\">https:\/\/go.nature.com\/2r4Smbu<\/a>)<\/li>\n<li><strong>Structure and Biocatalytic Scope of Coclaurine N-Methyltransferase.<\/strong> M. R. Bennett, M. L. Thompson, S. A. Shepherd, M. S. Dunstan, A. J. Herbert, D. R. M. Smith, V. A. Cronin, B. R. K. Menon, C. Levy &amp; J. Micklefield <em>Angew.<\/em> <em>Chem. Int.<\/em> <em>Ed.<\/em> <strong>2018, <\/strong><em>57<\/em>, 10600-10604. (<a href=\"http:\/\/dx.doi.org\/10.1002\/anie.201805060\">http:\/\/dx.doi.org\/10.1002\/anie.201805060<\/a>)<\/li>\n<li><strong>De novo Biosynthesis of &#8216;Non-Natural&#8217; Thaxtomin Phytotoxins. <\/strong>M. Winn, D. Francis &amp; J. Micklefield\u00a0 <em>Angew. Chem. Int. Ed. <\/em><strong>2018, <\/strong>57, 6830\u20136833 (<a href=\"http:\/\/dx.doi.org\/10.1002\/anie.201801525\">http:\/\/dx.doi.org\/10.1002\/anie.201801525<\/a>)<\/li>\n<li><strong>The Cycloaspeptides: Uncovering a new model for methylated nonribosomal peptide biosynthesis<\/strong>. K. M. J. de Mattos-Shipley, C. Greco, D. M. Heard, G. Hough, N. P. Mulholland, J. L. Vincent, J. Micklefield, T. J. Simpson, C. L. Willis, R. J. Cox and A. M. Bailey <em>Chem. Sci. <\/em><strong>2018<\/strong>, 9,\u00a04109-4117 (<a href=\"http:\/\/dx.doi.org\/10.1039\/C8SC00717A\">http:\/\/dx.doi.org\/10.1039\/C8SC00717A<\/a>)<\/li>\n<li><strong>Development of Halogenase Enzymes for Use in Synthesis <\/strong>J. Latham, E. Brandenburger, S. A. Shepherd, B. R. K. Menon and J.Micklefield <em>Chem. Rev<\/em> <strong>2018<\/strong>, <em>118<\/em>, 232-269;\u00a0 (<a href=\"http:\/\/dx.doi.org\/10.1021\/acs.chemrev.7b00032\">http:\/\/dx.doi.org\/10.1021\/acs.chemrev.7b00032<\/a>)<\/li>\n<li><strong>An automated Design-Build-Test-Learn pipeline for enhances microbial production of fine chemicals <\/strong>Carbonell, P. <em>et al <\/em><em>Communication Biology <\/em><strong>2018,<\/strong>\u00a0<em>1<\/em>, 66 (<a href=\"http:\/\/dx.doi.org\/10.1038\/s42003-018-0076-9\">http:\/\/dx.doi.org\/10.1038\/s42003-018-0076-9<\/a>)<\/li>\n<\/ul>\n<p>2017<\/p>\n<ul>\n<li><strong>RadH: A Versatile Halogenase for Integration into Synthetic Pathways. <\/strong>B. R. K. Menon, E. Brandenburger, H. H. Sharif, U. Klemstein, S.A. Shepherd, M. F. Greaney, and J. Micklefield\u00a0 <em>Angew. Chem. Int. Ed. <\/em><strong>2017<\/strong>, <em>56<\/em>, 11841 \u201311845 (<a href=\"http:\/\/dx.doi.org\/10.1002\/anie.201706342\">http:\/\/dx.doi.org\/10.1002\/anie.201706342<\/a>)<\/li>\n<li><span style=\"color: #000000;\"><b>From multi-step Enzyme Monitoring to Whole-Cell Biotransformations: development of real-time UVRR spectroscopy.\u00a0<\/b>H. Fisk, Y. Xu, C.Westley, N. Turner, J. Micklefield, R. Goodacre. <em>Analytical Chemistry\u00a0<\/em><strong>2017,\u00a0<\/strong><em>89,\u00a0<\/em>12527-12532<strong> (<\/strong><a href=\"http:\/\/pubs.acs.org\/doi\/10.1021\/acs.analchem.7b03742\">http:\/\/dx.doi.org\/10.1021\/acs.analchem.7b03742<\/a>)\u00a0\u00a0<\/span><\/li>\n<li><strong>Development of Halogenase Enzymes for use in Synthesis.\u00a0<\/strong>Jonathan Latham, Eileen Brandenburger, Sarah A. Shepherd, Binuraj R. K. Menon and Jason Micklefield.\u00a0<em>Chem. Rev.\u00a0<\/em>2017, in press<strong>\u00a0<\/strong>(<a href=\"http:\/\/pubs.acs.org\/articlesonrequest\/AOR-XJV72fJWeGVD7wpMYnnX\">DOI: 10.1021\/acs.chemrev.7b00032<\/a><\/li>\n<li><strong>Real-time monitoring of enzyme-catalysed reactions using deep UV resonance Raman spectroscopy <\/strong>C. Westley, H. Fisk, Y. Xu, K. A. Hollywood,\u00a0 A. J. Carnell, J. Micklefield, N. J. Turner, R. Goodacre <em>Chem. Eur. J., <\/em><strong>2017, <\/strong><em>23<\/em><strong>, <\/strong>6983\u20136987 (<a href=\"http:\/\/dx.doi.org\/10.1002\/chem.201701388\">http:\/\/dx.doi.org\/10.1002\/chem.201701388<\/a>)<\/li>\n<li><strong>Recent Advances in Methyltransferase Biocatalysis.\u00a0<\/strong>M.R. Bennett, S.A. Shepherd, V.A. Cronin, J. Micklefield <i>Current Opinion in Chemical Biology.\u00a0<\/i><strong>2017,\u00a0<\/strong><em>37,\u00a0<\/em>97-106<em>.\u00a0(<a href=\"http:\/\/dx.doi.org\/10.1016\/j.cbpa.2017.01.020\">http:\/\/dx.doi.org\/10.1016\/j.cbpa.2017.01.020<\/a>)<\/em><\/li>\n<li><strong><strong>An Engineered Tryptophan Synthase Opens New Enzymatic Pathways t<\/strong>o\u00a0\u03b2-Methyltryptophan and Derivatives.\u00a0<\/strong>Daniel Francis, Michael Winn, Jonathan Latham, Michael F Greaney and Jason Micklefield.\u00a0<em>ChemBioChem.\u00a0<\/em><strong>2017<\/strong>, 18, 382-386<strong> (<\/strong><a href=\"http:\/\/dx.doi.org\/10.1002\/cbic.201600471\">http:\/\/dx.doi.org\/10.1002\/cbic.201600471<\/a><strong>)<\/strong><\/li>\n<\/ul>\n<p>2016<\/p>\n<ul>\n<li><strong>Integrated Catalysis Opens New Arylation Pathways via Regiodivergent Enzymatic C-H Activation. <\/strong>J. Latham<em>, <\/em>J.-M. Henry, H. H. Sharif,\u00a0B. R. K. Menon, S. A. Shepherd,\u00a0 M. F. Greaney, J.Micklefield.\u00a0<em>Nature Commun. <\/em><strong>2016<\/strong>, 7, 11873 (<a href=\"http:\/\/dx.doi.org\/10.1038\/NCOMMS11873\">http:\/\/dx.doi.org\/10.1038\/NCOMMS11873<\/a>)<\/li>\n<li><strong>Rewiring Riboswitches to Create New Genetic Circuits in Bacteria.\u00a0<\/strong>C.J. Robinson, D. Medina-Stacey, M.-C. Wu, H.A. Vincent, J. Micklefield\u00a0<em>Methods in Enzymology\u00a0<\/em><strong>2016, <\/strong><em>575<\/em>, 319\u2013348.<em>(<a href=\"http:\/\/dx.doi.org\/10.1016\/bs.mie.2016.02.022\">http:\/\/dx.doi.org\/10.1016\/bs.mie.2016.02.022<\/a>)<\/em><\/li>\n<li><strong>A flavin-dependent decarboxylase-dehydrogenase-monooxygenase assembles the warhead of \u03b1, \u03b2-epoxyketone proteasome inhibitors.<\/strong> D.\u00a0Zabala; J. W. Cartwright; D. M. Roberts; B. J. C. Law; L. Song; M. Samborskyy; P. F. Leadlay; J. Micklefield; G. L. Challis <em>J. Am. Chem. Soc.<\/em> <strong>2016,<\/strong>\u00a0<em>138:\u00a04342\u20134345 (<a href=\"http:\/\/dx.doi.org\/10.1021\/jacs.6b01619\"><span style=\"color: #000000;\">http:\/\/dx.doi.org\/<\/span>10.1021\/jacs.6b01619<\/a>)<\/em><\/li>\n<li><strong>An Enzyme Cascade for Selective Modification of Tyrosine<br \/>\nResidues in Structurally Diverse Peptides and Proteins.\u00a0<\/strong>A.-W. Struck, M. R. Bennett, S. A. Shepherd, B. J. C. Law, Y. Zhuo, L. S. Wong, J. Micklefield\u00a0<em>J. Am. Chem. Soc.<\/em>\u00a0<strong>2016,<\/strong>\u00a0<em>138,\u00a0<\/em>3038-3045<strong>\u00a0<\/strong><em>\u00a0(<a href=\"http:\/\/dx.doi.org\/10.1021\/jacs.5b10928\">http:\/\/dx.doi.org\/10.1021\/jacs.5b10928<\/a>)\u00a0<\/em><\/li>\n<li><strong>A Structure-Guided Switch in the Regioselectivity<\/strong> <strong>of a Tryptophan Halogenase. <\/strong>S. A. Shepherd, B. R. K. Menon, H. Fisk, A.-W. Struck, C. Levy, D. Leys, J. Micklefield\u00a0<em>ChemBioChem<\/em> <strong>2016,\u00a0<\/strong>17, 821\u2013824 \u00a0<a href=\"http:\/\/dx.doi.org\/10.1002\/cbic.201600051\">(<\/a><a href=\"http:\/\/dx.doi.org\/10.1002\/cbic.201600051\">http:\/\/dx.doi.org\/10.1002\/cbic.201600051<\/a><a href=\"http:\/\/dx.doi.org\/10.1002\/cbic.201600051\">)<\/a><\/li>\n<li><strong>Recent Advances in Engineering Non-Ribosomal Peptide Assembly Lines. <\/strong>M. Winn, J. Fyans, Y. Zhuo, J. Micklefield <em>Nat. Prod. Rep<\/em>.<strong> 2016,<\/strong> <em>33,\u00a0<\/em>317-347<strong>\u00a0<\/strong><em>\u00a0<\/em>(<a href=\"http:\/\/dx.doi.org\/10.1039\/C5NP00099H\">http:\/\/dx.doi.org\/10.1039\/C5NP00099H<\/a>)<\/li>\n<li><strong>Effects of Active Site Modification and Quaternary Structure on the Regioselectivity of Catechol-<em>O<\/em>-Methyltransferase. <\/strong>B. J. C. Law, M. R. Bennett, M. L. Thompson, C. Levy, S. A. Shepherd, D. Leys, and J. Micklefield <em>Angew. Chem. Int. Ed. <\/em><strong>2016,\u00a0<\/strong><em>55: 2683-2687<\/em>. (<a href=\"http:\/\/dx.doi.org\/10.1002\/anie.201508287\">http:\/\/dx.doi.org\/<span class=\"article-header__meta-info-data\">10.1002\/anie.201508287<\/span><\/a>)<\/li>\n<li><strong>Dual transcriptional-translational cascade permits cellular level tuneable expression control.\u00a0<\/strong>R. Morra, J. Shankar, C.J.Robinson, L. Butler, S. Halliwell, S. Hay, J. Micklefield, N. Dixon\u00a0<em>Nucleic Acid Res.\u00a0<\/em><strong>2016,\u00a0<\/strong><em>44, e21 (<a href=\"http:\/\/dx.doi.org\/10.1093\/nar\/gkv912\">http:\/\/dx.doi.org\/10.1093\/nar\/gkv912<\/a>)<\/em><em>\u00a0<\/em><\/li>\n<li><strong>SYNBIOCHEM Synthetic Biology Research Centre, Manchester &#8211; A UK foundry for fine and speciality chemicals production.\u00a0<\/strong>Le Feuvre R. A.; Carbonell P.; Currin, A.; Dunstan, M.; Fellows, D.; Jervis, A. J.; Rattray, N. J. W.; Robinson, C. J.; Swainston, N.; Vinaixa, M.; Williams, A.; Yan, C.; Barran, P.; Breitling, R.; Chen, G. G.; Faulon, J. L.; Goble, C.; Goodacre, R.; Kell, D. B.; Micklefield, J.; Scrutton, N. S.; Shapira, P; Takano, E.; Turner, N. J.; <em>Synthetic and Systems Biotechnology<\/em> 1 <strong>2016<\/strong>, 271e275 (<a href=\"http:\/\/dx.doi.org\/10.1016\/j.synbio.2016.07.001\">http:\/\/dx.doi.org\/10.1016\/j.synbio.2016.07.001<\/a>)<\/li>\n<li><strong>SYNBIOCHEM-a SynBio foundry for the biosynthesis and sustainable production of fine and speciality chemicals<\/strong>. Carbonell P , Currin A , Dunstan M , Fellows D , Jervis A , Rattray NJ , Robinson CJ , Swainston N , Vinaixa M , Williams A , Yan C , Barran P , Breitling R , Chen GG , Faulon JL , Goble C , Goodacre R , Kell DB , Feuvre RL , Micklefield J , Scrutton NS , Shapira P , Takano E , Turner\u00a0\u00a0<em>Biochemical Society Transactions<\/em> <strong>2016<\/strong>, <em>44<\/em>, 675-677 (<a href=\"http:\/\/dx.doi.org\/10.1042\/BST20160009\">http:\/\/dx.doi.org\/10.1042\/BST20160009<\/a>)<\/li>\n<li><strong>Changing the Selectivity of Biocatalysts.\u00a0<\/strong>M. Campbell, J. Micklefield\u00a0<em>ChemViews,\u00a0<\/em><strong>2016,\u00a0<\/strong><em>05, April \u00a0<\/em>(<a href=\"http:\/\/dx.doi.org\/10.1002\/chemv.201600027\">http:\/\/dx.doi.org\/10.1002\/chemv.201600027<\/a>) (Magazine article)<\/li>\n<li><strong>Structure and Biocatalytic Scope of Thermophillic Flavin-Dependent Halogenase and Flavin Reductase Enzymes <\/strong>B. R. K. Menon, J. Latham, M. S. Dunstan, E. Brandenburger, U. Klemstein, S. A. Shepherd, C. Karthikeyan, M. F. Greaney, D. Leys and J. Micklefield* <em>Org. Biomol. Chem.<\/em> <strong>2016<\/strong>, <em>14<\/em>, 9354\u20139361<strong>.<\/strong> (<a href=\"http:\/\/dx.doi.org\/10.1039\/C6OB01861K\">http:\/\/dx.doi.org\/10.1039\/C6OB01861K<\/a>)<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p>2015<\/p>\n<ul>\n<li><strong>Rational Re-engineering of the Transcriptional Silencing PreQ<sub>1<\/sub> Riboswitch. <\/strong>M.-C. Wu, P. T. Lowe, C. J. Robinson, H. A. Vincent, N. Dixon, J. Leigh,\u00a0 J. Micklefield <em>J. Am. Chem. Soc.<\/em> <strong>2015,\u00a0<\/strong><em>137,\u00a0<\/em>9015-9021<em>\u00a0(<\/em><a href=\"http:\/\/pubs.acs.org\/doi\/abs\/10.1021\/jacs.5b03405\">http:\/\/dx.doi.org\/10.1021\/jacs.5b03405<\/a>)<\/li>\n<li><strong>Extending the Biocatalytic Scope of Regiocomplementary Flavin-Dependent Halogenase Enzymes. <\/strong>S. A. Shepherd, C. Karthikeyan, J. Latham, A.-W. Struck, M. L. Thompson, B. Menon, M. Styles, C. Levy, D. Leys and J. Micklefield\u00a0<em>Chemical Science <\/em><strong>2015,\u00a0<\/strong><em>6, <\/em>3454-3460<em>\u00a0<\/em>(<a href=\"http:\/\/pubs.rsc.org\/en\/Content\/ArticleLanding\/2015\/SC\/C5SC00913H#!divAbstract\">http:\/\/dx.doi.org\/10.1039\/C5SC00913H<\/a>)<\/li>\n<li><strong>Engineered Biosynthesis of Enduracidin Lipogyclopeptide Antibiotics using the Ramoplanin Mannosyltransferase Ram29. <\/strong>M.-C. Wu, M. Q. Styles, B. J. C. Law, A. W. Struck, L. Nunns and J. Micklefield\u00a0<em>Microbiology<\/em> <strong>2015,<\/strong>\u00a0<em>161: 1338-1347<\/em><em>\u00a0<\/em>DOI:<a href=\"http:\/\/dx.doi.org\/10.1099\/mic.0.000095\">10.1099\/mic.0.000095<\/a><\/li>\n<li><strong>Functional Exchangeability of Oxidase and Dehydrogenase Reactions in the Biosynthesis of Hydroxyphenylglycine, a Nonribosomal Peptide Building Block. <\/strong>V. Diez, M. Loznik, S. Taylor, M. Winn, N. J. W. Rattray, H. Podmore, J. Micklefield, R. Goodacre, M. H. Medema, U. M\u00fcller, R. Bovenberg, D. B. Janssenand E. Takano\u00a0<em>ACS synthetic Biology<\/em><strong> 2015<\/strong><em>, 4,<\/em>\u00a0796-807.<em>\u00a0\u00a0<\/em>DOI:<a href=\"http:\/\/dx.doi.org\/10.1021\/sb500368w\">10.1021\/sb500368w<\/a><\/li>\n<li><strong>Site-specific bioalkylation of Rapamycin by the RapM 16-<em>O<\/em>-methyltransferase. <\/strong>B. J. C. Law, A.-W. Struck, M. R. Bennett, B. Wilkinson and J. Micklefield\u00a0<em>Chemical Science <\/em><strong>2015,\u00a0<\/strong><em>6, <\/em>2885-2892<em>\u00a0<\/em>DOI:<a href=\"http:\/\/dx.doi.org\/10.1039\/C5SC00164A\"><span style=\"text-decoration: underline;\">10.1039\/C5SC00164A<\/span><\/a><\/li>\n<li><strong>Enzymatic<\/strong> <strong>Enantioselective Decarboxylative Protonation of<\/strong><strong> Heteroaryl Malonates.<\/strong> R. Lewin, M. Goodall, M. L.Thompson, J. Leigh, M. Breuer, K. Baldenius and J. Micklefield\u00a0<em>Chem. Eur. J., <\/em><strong>2015, <\/strong><em>17<\/em>, 6557-6563 DOI:<a href=\"http:\/\/dx.doi.org\/10.1002\/chem.201406014\">10.1002\/chem.201406014<\/a><\/li>\n<li><strong>Minimum Information about a Biosynthetic Gene cluster (MIBiG) specification. <\/strong>M. H. Medema et al.\u00a0<em>Nature Chem. Biol.<\/em> <strong>2015<\/strong>, <em>11<\/em>, 625\u2013631. (<a href=\"http:\/\/dx.doi.org\/10.1038\/nchembio.1890\">http:\/\/dx.doi.org\/10.1038\/nchembio.1890<\/a>)<\/li>\n<\/ul>\n<p>2014<\/p>\n<ul>\n<li><strong>Enzymatic Carboxylation and Decarboxylation<\/strong>. R. Lewin, M. Thompson and J. Micklefield\u00a0<em>Science of Synthesis: Biocatalysis in Organic Synthesis,<\/em> <strong>2014<\/strong>, 2, 133-157<\/li>\n<li><strong>A High-Throughput Assay for Arylamine Halogenation Based on a Peroxidase-Mediated Quinone-Amine Coupling with Applications in the Screening of Enzymatic Halogenations.\u00a0<\/strong>J. Hosford, S.A. Shepherd, J. Micklefield, L. S. Wong<em>\u00a0Chem. Eur. J., <\/em><strong>2014<\/strong>, <em>20, <\/em>16759-16763 DOI:<a href=\"http:\/\/dx.doi.org\/10.1002\/chem.201403953\">10.1002\/chem.201403953<\/a><\/li>\n<li><strong>Modular Riboswitch Toolsets for Synthetic Genetic Control in Diverse Bacterial Species<\/strong><strong>. <\/strong>C. J. Robinson, H. A. Vincent, M.-C. Wu, P. T. Lowe, M. S. Dunstan,D. Leys,<sup>\u00a0<\/sup>and J. Micklefield\u00a0<em>J. Am. Chem. Soc.<\/em><strong> 2014<\/strong>,<em> 136, <\/em>10615-10624<em>. <\/em>DOI:<a href=\"http:\/\/dx.doi.org\/10.1021\/ja502873j\">10.1021\/ja502873j<\/a><\/li>\n<li><strong>Generation of Orthogonally Selective Bacterial Riboswitches by Targeted Mutagenesis and <em>in vivo<\/em> Screening. <\/strong>H. Vincent, C. Robinson, M.-C Wu, N. Dixon and J. Micklefield\u00a0<em>Methods in Molecular Biology<\/em> <strong>2014<\/strong>, <em>1111, <\/em>107-129\u00a0 DOI:<a href=\"http:\/\/dx.doi.org\/10.1007\/978-1-62703-755-6_8\">10.1007\/978-1-62703-755-6_8<\/a><\/li>\n<\/ul>\n<p>2013<\/p>\n<ul>\n<li><strong>Nucleic acids: new life, new materials. <\/strong>M. J. Gait, M. Komiyama, N. C. Seeman, O. Seitz, J. Micklefield and D. R. Liu<em> Org. Biomol. Chem. <\/em><strong>2013<\/strong><em>, 11, <\/em>2058-2059 (commentary). DOI:<a href=\"http:\/\/dx.doi.org\/10.1039\/C3OB90017G\">10.1039\/C3OB90017G<\/a><\/li>\n<\/ul>\n<p>2012<\/p>\n<ul>\n<li><strong>Probing riboswitch-ligand interactions using thiamine pyrophosphate analogues.<\/strong>\u00a0Chen L, Cressina E, Dixon N, Erixon K, Agyei-Owusu K, Micklefield J, Smith A, Abell C, Leeper F. \u00a0<em>Org. Biomol. Chem<\/em>.,\u00a0<strong>2012,<\/strong>\u00a0<em>10<\/em>, 5924-5931. DOI:<a href=\"http:\/\/dx.doi.org\/10.1039\/c2ob07116a\">10.1039\/c2ob07116a<\/a><\/li>\n<li><strong>Thermal Bifunctionality of Bacterial Phenylalanine Aminomutase and Ammonia Lyase Enzymes.\u00a0<\/strong>Chesters C, Wilding M, Goodall M, Micklefield J. \u00a0<em>Angew. Chem. Int. Ed. Engl<\/em>.,\u00a0<strong>2012,<\/strong>\u00a0<em>51<\/em>, 4344-4348.\u00a0\u00a0DOI:<a href=\"http:\/\/dx.doi.org\/10.1002\/anie.201200669\">10.1002\/anie.201200669<\/a><\/li>\n<li><strong>Orthogonal riboswitches for tuneable coexpression in bacteria.\u00a0<\/strong>Dixon N, Robinson C, Geerlings T, Duncan J, Drummond S, Micklefield J. <em>Angew. Chem. Int. Ed. Engl<\/em>.,\u00a0<strong>2012<\/strong>,\u00a0<em>51<\/em>(15), 3620-4. DOI:<a href=\"http:\/\/dx.doi.org\/10.1002\/anie.201109106\">10.1002\/anie.201109106<\/a><\/li>\n<li><strong>Introduction of a non-natural amino Acid into a nonribosomal Peptide antibiotic by modification of adenylation domain specificity.\u00a0<\/strong>Thirlway J, Lewis R, Nunns L, Al Nakeeb M, Styles M, Struck A, Smith C, Micklefield J. \u00a0<em>Angew. Chem. Int. Ed. Engl<\/em>.,\u00a0<strong>2012,<\/strong>\u00a0<em>51<\/em>(29): DOI:<a href=\"http:\/\/dx.doi.org\/10.1002\/anie.201202043\">10.1002\/anie.201202043<\/a><\/li>\n<li><strong>Decarboxylases: Enzymatic enantioselective decarboxylative protonation and C-C bond formation. <\/strong>Wilding M, Goodall M, Micklefield J. in Comprehensive Chirality, H. Yamamoto &amp; E. Carreira (Eds.). <strong>2012,<\/strong> January: eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:161723\">161723<\/a><\/li>\n<li><strong>A methodology for preparing nanostructured biomolecular interfaces with high enzymatic activity.\u00a0<\/strong>Wong L, Karthikeyan C, Eichelsdoerfer D, Micklefield J, Mirkin C. \u00a0<em>Nanoscale<\/em>,\u00a0<strong>2012,<\/strong>\u00a0<em>4<\/em>(2), 659-66. DOI:<a href=\"http:\/\/dx.doi.org\/10.1039\/c1nr11443c\">10.1039\/c1nr11443c<\/a><\/li>\n<li><strong>Bioengineering natural product biosynthetic pathways for therapeutic applications.\u00a0<\/strong>Wu M, Law B, Wilkinson B, Micklefield J. \u00a0<em>Curr. Opin. Biotechnol<\/em>., in press.\u00a0<strong>2012,<\/strong>\u00a0DOI:<a href=\"http:\/\/dx.doi.org\/10.1016\/j.copbio.2012.03.008\">10.1016\/j.copbio.2012.03.008<\/a><\/li>\n<\/ul>\n<p>2011<\/p>\n<ul>\n<li><strong>Borrelidin modulates the alternative splicing of VEGF in favour of anti-angiogenic isoforms.\u00a0<\/strong>Woolard, J.; Vousden, W.; Moss, S. J.; Krishnakumar, A.; Gammons, M. V. R.; Nowak, D. G.; Dixon, N.; Micklefield, J.; Spannhoff, A.; Bedford, M. T.; Gregory, M. A.; Martin, C. J.; Leadlay, P. F.; Zhang, M. Q.; Harper, S. J.; Bates, D. O.; Wilkinson, B. \u00a0<em>Chem Sci<\/em>,\u00a0<strong>2011,<\/strong>\u00a0<em>2<\/em>, 273-278. \u00a0DOI:<a href=\"http:\/\/dx.doi.org\/10.1039\/C0SC00297F\">10.1039\/C0SC00297F<\/a><\/li>\n<li><strong>Protein micro- and nanopatterning using aminosilanes with protein-resistant photolabile protecting groups.\u00a0<\/strong>Alang Ahmad S, Wong L, Ul-Haq E, Hobbs J, Leggett G, Micklefield J. \u00a0<em>J. Am. Chem. Soc<\/em>.,\u00a0<strong>2011,<\/strong>\u00a0<em>133<\/em>(8), 2749-59.\u00a0\u00a0DOI:<a href=\"http:\/\/dx.doi.org\/10.1021\/ja1103662\">10.1021\/ja1103662<\/a><\/li>\n<li><strong>Active site modification of the \u03b2-ketoacyl-ACP synthase FabF3 of Streptomyces coelicolor affects the fatty acid chain length of the CDA lipopeptides.\u00a0<\/strong>Lewis R, Nunns L, Thirlway J, Carroll K, Smith C, Micklefield J. \u00a0<em>Chem. Commun. (Camb.)<\/em>,\u00a0<strong>2011,<\/strong>\u00a0<em>47<\/em>(6), 1860-2. DOI:<a href=\"http:\/\/dx.doi.org\/10.1039\/c0cc03444d\">10.1039\/c0cc03444d<\/a><\/li>\n<li><strong>Biophysical and cellular-uptake properties of mixed-sequence pyrrolidine-amide oligonucleotide mimics.\u00a0<\/strong>Worthington R, Micklefield <em>J. \u00a0Chem. Eur. J<\/em>. <strong>2011,<\/strong> December; 17(51): 14429-14441. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:147585\">147585<\/a>\u00a0| PMID:<a href=\"http:\/\/www.pubmed.org\/22076794\">22076794<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1002\/chem.201101950\">10.1002\/chem.201101950<\/a><\/li>\n<li><strong>The Snomipede: A Parallel Platform for Scanning Near-Field Photolithography.\u00a0<\/strong>ul-Haq E, Liu Z, Wong L, Hobbs J, Leggett G, Micklefield J, Roberts C, Weaver J. \u00a0<em>J Mater Res<\/em>.,<strong>\u00a02011,<\/strong><em>\u00a026<\/em>, 2997-3008.\u00a0\u00a0DOI:<a href=\"http:\/\/dx.doi.org\/10.1557\/jmr.2011.370\">10.1557\/jmr.2011.370<\/a><\/li>\n<\/ul>\n<p>2010<\/p>\n<ul>\n<li><strong>A non-enzymatic, DNA template-directed morpholino primer extension approach<\/strong>. Bell N, Wong R, Micklefield J. <em>Chem. Eur. J<\/em>. \u00a0<strong>2010,<\/strong> February; 16(7): 2026-2030. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:77990\">77990<\/a>\u00a0| PMID:<a href=\"http:\/\/www.pubmed.org\/20087911\">20087911<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1002\/chem.200902237\">10.1002\/chem.200902237<\/a><\/li>\n<li><strong>Reengineering orthogonally selective riboswitches.\u00a0<\/strong>Dixon N, Duncan J, Geerlings T, Dunstan M, McCarthy J, Leys D, Micklefield J. \u00a0<em>Proc. Natl. Acad. Sci. U.S.A<\/em>.\u00a0<strong>2010,<\/strong>\u00a0<em>107(7),<\/em>\u00a02830-2835. DOI:<a href=\"http:\/\/dx.doi.org\/10.1073\/pnas.0911209107\">10.1073\/pnas.0911209107<\/a><\/li>\n<li><strong>Parallel Scanning Near-Field Photolithography: The Snomipede. <\/strong>Haq E, Liu Z, Zhang Y, Ahmad S, Wong L, Armes S, Hobbs J, Leggett G, Micklefield J, Roberts C, Weaver J. <em>Nano Letters<\/em>. <strong>2010,<\/strong> October; 10: 4375-4380. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:100523\">100523<\/a>\u00a0| PMID:<a href=\"http:\/\/www.pubmed.org\/20945880\">20945880<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1021\/nl1018782\">10.1021\/nl1018782<\/a><\/li>\n<li><strong>Nanoscale biomolecular structures on self-assembled monolayers generated from modular pegylated disulfides.<\/strong>Wong L, Janusz S, Sun S, Leggett G, Micklefield J. <em>Chem. Eur. J<\/em>. \u00a0<strong>2010,<\/strong> October; 16(40): 12234-43. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:100522\">100522<\/a>\u00a0| PMID:<a href=\"http:\/\/www.pubmed.org\/20839371\">20839371<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1002\/chem.200902439\">10.1002\/chem.200902439<\/a><\/li>\n<li><strong>Site-selective immobilisation of functional enzymes on to polystyrene nanoparticles. <\/strong>Wong L, Okrasa K, Micklefield J. <em>Org. Biomol. Chem<strong>.<\/strong><\/em><strong> 2010,<\/strong>\u00a08(4): 782-787. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:77988\">77988<\/a>\u00a0| PMID:<a href=\"http:\/\/www.pubmed.org\/20135034\">20135034<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1039\/b916773k\">10.1039\/b916773k<\/a><\/li>\n<\/ul>\n<p>2009<\/p>\n<ul>\n<li><strong>Selective Covalent Protein Immobilization: Strategies and Applications.<\/strong> L. S. Wong, F. Khan, J. Micklefield. <em>Chem. Rev<\/em>. <strong>2009,<\/strong>\u00a0109: 4025-4053. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:12503\">12503<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1021\/cr8004668\">10.1021\/cr8004668<\/a><\/li>\n<li><strong>Micrometer- and nanometer-scale photopatterning using 2-nitrophenylpropyloxycarbonyl-protected aminosiloxane monolayers.<\/strong> Ahmad S, Wong L, ul-Haq E, Hobbs J, Leggett G, Micklefield J. <em>J. Am. Chem. Soc<\/em>. <strong>2009,<\/strong>\u00a0131(4): 1513-1522. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:18292\">18292<\/a>\u00a0| PMID:<a href=\"http:\/\/www.pubmed.org\/19173668\">19173668<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1021\/ja807612y\">10.1021\/ja807612y<\/a><\/li>\n<li><strong>Chemical Modification of Oligonucleotides for Therapeutic, Bioanalytical and other Applications.<\/strong> Bell N, Micklefield J. <em>Chembiochem : A European Journal of Chemical Biology<\/em>. <strong>2009,<\/strong> September; 17: 2691-2703. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:18265\">18265<\/a>\u00a0| PMID:<a href=\"http:\/\/www.pubmed.org\/19739190\">19739190<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1002\/cbic.200900341\">10.1002\/cbic.200900341<\/a><\/li>\n<li><strong>Lipase-catalysed kinetic resolutions of secondary alcohols in pressurised liquid hydrofluorocarbons. <\/strong>Corr S, Ball A, Micklefield J. <em>Tetrahedron Lett<\/em>. <strong>2009,<\/strong>\u00a0(50): 3543-3546. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:18269\">18269<\/a>\u00a0| PMID:<a href=\"http:\/\/www.pubmed.org\/19739190\">19739190<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1016\/j.tetlet.2009.03.037\">10.1016\/j.tetlet.2009.03.037<\/a><\/li>\n<li><strong>Biosynthesis and biosynthetic engineering of calcium-dependent lipopeptide antibiotics.<\/strong> Micklefield J. <em>Pure Appl. Chem<\/em>. <strong>2009,<\/strong>\u00a081: 1065-1074. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:18270\">18270<\/a>\u00a0| PMID:<a href=\"http:\/\/www.pubmed.org\/19739187\">19739187<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1351\/PAC-CON-08-08-29\">10.1351\/PAC-CON-08-08-29<\/a><\/li>\n<li><strong>Structure-Guided Directed Evolution of Alkenyl and Arylmalonate Decarboxylases. <\/strong>Okrasa K, Levy C, Wilding M, Goodall M, Baudendistel N, Hauer B, Leys D, Micklefield J. <em>Angew. Chem. Int. Ed. Engl<\/em>. <strong>2009,<\/strong>\u00a048: 7691-7694. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:13316\">13316<\/a>\u00a0| PMID:<a href=\"http:\/\/www.pubmed.org\/19739187\">19739187<\/a>| DOI:<a href=\"http:\/\/dx.doi.org\/10.1002\/anie.200904112\">10.1002\/anie.200904112<\/a><\/li>\n<li><strong>Biosynthesis of nonribosomal peptide precursors. <\/strong>Wilkinson B, Micklefield J. <em>Meth. Enzymol<\/em>. <strong>2009,<\/strong>\u00a0458: 353-378. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:18266\">18266<\/a>\u00a0| PMID:<a href=\"http:\/\/www.pubmed.org\/19374990\">19374990<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1016\/S0076-6879(09)04814-9\">10.1016\/S0076-6879(09)04814-9<\/a><\/li>\n<\/ul>\n<p>2008<\/p>\n<ul>\n<li><strong>Auxotrophic-precursor directed biosynthesis of nonribosomal lipopeptides with modified tryptophan residues.<\/strong> B. Amir-Heidari, J. thirlway and J. Micklefield. <em>Org. Biomol. Chem<\/em>. <strong>2008,<\/strong>\u00a06: 975-978. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a6714\">1a6714<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1039\/b718766c\">10.1039\/b718766c<\/a><\/li>\n<li><strong>Combinatorial biosynthesis of nonribosomal lipopeptides. <\/strong>J. Micklefield. <em>Chemica Oggi<\/em>. <strong>2008,<\/strong> January; 26: 6-8. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a12240\">1a12240<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1002\/chin.200949266\">10.1002\/chin.200949266<\/a><\/li>\n<li><strong>Direct site-selective covalent protein immobilization catalyzed by a phosphopantetheinyl transferase. <\/strong>L. S. Wong, J. Thirlway and J. Micklefield. <em>J. Am. Chem. Soc<\/em>. <strong>2008,<\/strong>\u00a0130: 12456-12464. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:17302\">17302<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1021\/ja8030278\">10.1021\/ja8030278<\/a><\/li>\n<li><strong>Natural products hit the targets other molecules cannot reach. <\/strong>J. Micklefield. <em>Chemical Biology<\/em>. <strong>2008,<\/strong>\u00a03: 7-7. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a6731\">1a6731<\/a><\/li>\n<li><strong>New Malonate Decarboxylases for Industrial Applications <\/strong>K. Okrasa, C. Levy, N. Baudistel, B. Hauer, D. Leys and J. Micklefield. <em>PCT Int. Appl.<\/em> WO 2009068308 <strong>2008,<\/strong>\u00a0eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:13a38\">13a38<\/a><\/li>\n<li><strong>RNA selective cross-pairing of backbone extended pyrrolidine-amide oligonucleotide mimics (bePOMs). <\/strong>R. Worthington, N. Bell and J. Micklefield. <em>Org. Biomol. Chem<\/em>. <strong>2008,<\/strong> January; 6: 92-103. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a6716\">1a6716<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1039\/b714580m\">10.1039\/b714580m<\/a><\/li>\n<li><strong>Structure and mechanism of an unusual malonate decarboxylase and related racemases. <\/strong>K. Okrasa, C. Levy, N. Baudistel, D. Leys and J. Micklefield. <em>Chem. Eur. J<\/em>. <strong>2008,<\/strong>\u00a06: 6609-6613. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a12241\">1a12241<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1002\/chem.200800918\">10.1002\/chem.200800918<\/a><\/li>\n<li><strong>Subsurface biomolecular imaging of Streptomyces coelicolor using mass spectrometry.<\/strong> S. Vaidyanthan, J. S. Fletcher, N. P. Lockyer, R. Goodacre, J. Micklefield and J. C. Vickerman. <em>Anal. Chem<\/em>. <strong>2008,<\/strong>\u00a080: 1942-1951. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a6715\">1a6715<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1021\/ac701921e\">10.1021\/ac701921e<\/a><\/li>\n<\/ul>\n<p>2007<\/p>\n<ul>\n<li><strong>An asparagine oxygenase (AsnO) and a 3-hydroxyasparaginyl phosphotransferase (HasP) are involved in the biosynthesis of calcium-dependent lipopeptide antibiotics. <\/strong>J. Neary, A. Powell, L. Gordon, C. Milne, F. Flett, B. Wilkinson, C.P. Smith and J. Micklefield. <em>Microbiology<\/em>. <strong>2007,<\/strong>\u00a0153: 768-776. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a771\">1a771<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1099\/mic.0.2006\/002725-0\">10.1099\/mic.0.2006\/002725-0<\/a><\/li>\n<li><strong>Cellular targets of natural products. <\/strong>N. Dixon, L. S. Wong, T. Geerlings and J. Micklefield. <em>Nat. Prod. Rep<\/em>. <strong>2007,<\/strong>\u00a024: 1288-1310. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a6733\">1a6733<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1039\/b616808f\">10.1039\/b616808f<\/a><\/li>\n<li><strong>Engineered biosynthesis of nonribosomal lipopeptide antibiotics with modified fatty acid side chains. <\/strong>A. Powell, M. Borg B. Amir-Heidari, J. M. Neary, J. Thirlway, B. Wilkinson, C.P. Smith and J. Micklefield. <em>J. Am. Chem. Soc<\/em>. <strong>2007,<\/strong>\u00a0129: 15182-15191. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a6717\">1a6717<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1021\/ja074331o\">10.1021\/ja074331o<\/a><\/li>\n<li><strong>Homopolymeric pyrrolidine-amide oligonucleotide mimics: Fmoc-synthesis and DNA\/RNA binding properties. <\/strong>S.T.H. Tan, R. Worthington, A. O&#8217;Rourke J. Morral R. G. Pritchard and J. Micklefield. <em>Org. Biomol. Chem<\/em>. \u00a0<strong>2007,<\/strong>\u00a05: 239-248. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a6722\">1a6722<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1039\/b613384n\">10.1039\/b613384n<\/a><\/li>\n<li><strong>Mining and engineering natural-product biosynthetic pathways.<\/strong> B. Wilkinson and J. Micklefield. <em>Nat. Chem. Biol<\/em>.. <strong>2007,<\/strong>\u00a03: 379-386. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a6732\">1a6732<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1038\/nchembio.2007.7\">10.1038\/nchembio.2007.7<\/a><\/li>\n<li><strong>Mixed-sequence pyrrolidine-amide oligonucleotide mimics: Boc(Z) synthesis and DNA\/RNA binding properties. <\/strong>R. Worthington, A. O&#8217;Rourke J. Morral, S.T.H. Tan and J. Micklefield. <em>Org. Biomol. Chem<\/em>. <strong>2007,<\/strong>\u00a05: 249-259. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a772\">1a772<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1039\/b613386j\">10.1039\/b613386j<\/a><\/li>\n<li><strong>NMR confirmation that tryptophan dehydrogenation occurs with syn-stereochemistry during the biosynthesis of CDA in Streptomyces coelicolor. <\/strong>B. A. Heidari and J. Micklefield. <em>J. Org. Chem<\/em>. <strong>2007,<\/strong>\u00a072: 8950-8953. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a6719\">1a6719<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1021\/jo701660v\">10.1021\/jo701660v<\/a><\/li>\n<li><strong>Precursor-directed biosynthesis of nonribosomal lipopeptides with modified glutamate residues. <\/strong>A. Powell, M. Al Nakeeb, B. Wilkinson and J. Micklefield. <em>Chem. Commun<\/em>. <strong>2007,<\/strong>\u00a02683-2685. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a6720\">1a6720<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1039\/b706224a\">10.1039\/b706224a<\/a><\/li>\n<li><strong>Process for dynamic kinetic resolution (DKR) of racemic compounds in hydrofluorocarbon solvents. <\/strong>J. Micklefield, A. J. Ball and S. Corr. <em>PCT Int. Appl.<\/em> WO 2007129018 <strong>2007,<\/strong>\u00a0eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:13a39\">13a39<\/a><\/li>\n<li><strong>Stereochemical course of tryptophan dehydrogenation during biosynthesis of the calcium-dependent lipopeptide antibiotics. <\/strong>B. Amir-Heidari, J. Thirlway, and Jason Micklefield. <em>Org. Lett<\/em>. <strong>2007,<\/strong>\u00a09 : 1513-1516. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:17444\">17444<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1021\/ol0701619\">10.1021\/ol0701619<\/a><\/li>\n<li><strong>Stereospecific enzymatic transformation of \u03b1-ketoglutarate to (2S,3R)-3-methyl glutamate during acidic lipopeptide biosynthesis. <\/strong>C. Mahlert, F.Kopp, J. Thirlway, J. Micklefield, and M. A. Marahiel. <em>J. Am. Chem. Soc<\/em>. <strong>2007,<\/strong>\u00a0129: 12011-12018. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a6718\">1a6718<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1021\/ja074427i\">10.1021\/ja074427i<\/a><\/li>\n<\/ul>\n<p>2006<\/p>\n<ul>\n<li><strong>Biosynthesis of the (2S,3R)-3-Methyl Glutamate Residue of Nonribosomal Lipopeptides. <\/strong>C. Milne, A. Powell, J. Jim, M. Al Nakeeb C. P. Smith and J. Micklefield. <em>J. Am. Chem. Soc<\/em>. <strong>2006,<\/strong> August; 128: 11250-11259. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a9548\">1a9548<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1021\/ja062960c\">10.1021\/ja062960c<\/a><\/li>\n<li><strong>Effects of stereospecific backbone methylation on the DNA\/RNA binding properties of pyrrolidine-amide oligonucleotide mimics (cPOM). <\/strong>A. Kahn and J. Micklefield. <em>Chem. Commun<\/em>. <strong>2006,<\/strong>\u00a01436-1438. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a524\">1a524<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1039\/b518171b\">10.1039\/b518171b<\/a><\/li>\n<\/ul>\n<p>2005<\/p>\n<ul>\n<li><strong>Engineering the biosynthesis of nonribosomal lipopeptide antibiotics. <\/strong>C.P. Smith and J. Micklefield. In: M. G. Hicks &amp; C. Kettner, ed. The Chemical Theatre of Biological Systems (Logos Verlag-Berlin). Berlin, Logos Verlag-Berlin.<strong>2005,<\/strong>\u00a0eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:36107\">36107<\/a><\/li>\n<li><strong>Nature&#8217;s protection racket. <\/strong>J. Micklefield. <em>Chemistry and Biology<\/em>. <strong>2005,<\/strong>\u00a012: 1-3. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a6735\">1a6735<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1016\/j.chembiol.2005.06.002\">10.1016\/j.chembiol.2005.06.002<\/a><\/li>\n<li><strong>Reagents for carbonyl methylenation in organic synthesis. <\/strong><em>Curr. Org. Synth<\/em>. I. G. Beadham and J. Micklefield. <strong>2005,<\/strong>\u00a02: 231-259. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a6734\">1a6734<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.2174\/1570179053545396\">10.2174\/1570179053545396<\/a><\/li>\n<\/ul>\n<p>2004<\/p>\n<ul>\n<li><strong>A dynamic combinatorial screen for novel imine reductase activity. \u00a0<\/strong>H. Li, P. Williams, J. Micklefield, J. M. Gardiner and G. Stephens. <em>Tetrahedron<\/em>. <strong>2004,<\/strong>\u00a060: 753-758. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a4487\">1a4487<\/a><\/li>\n<li><strong>Active-site modifications of adenylation domains lead to hydrolysis of upstream nonribosomal peptidyl thioester intermediates. <\/strong>MICKLEFIELD J; UGURU GABRIEL C.; MILNE CLAIRE; BORG MATTHEW; . <em>J. Am. Chem. Soc<\/em>. <strong>2004,<\/strong> April; 126(16): 5032-5033. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a9549\">1a9549<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1021\/ja048778y\">10.1021\/ja048778y<\/a><\/li>\n<li><strong>Biotransformations in low-boiling hydrofluorocarbon solvents.<\/strong> MICKLEFIELD J; SAUL, SIMON; CORR, STUART. <em>Angew. Chem<\/em>. <strong>2004,<\/strong> October; 43(41): 5519-5523. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a9550\">1a9550<\/a>\u00a0| DOI:<a href=\"http:\/\/dx.doi.org\/10.1002\/anie.200460082\">10.1002\/anie.200460082<\/a><\/li>\n<li><strong>Daptomycin Structure and Mechanism of Action Revealed. <\/strong>J. Micklefield. <em>Chemistry &amp; Biology<\/em>. <strong>2004,<\/strong>\u00a011: 887-888. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a4604\">1a4604<\/a><\/li>\n<li><strong>Enzymatic process for stereo-selective preparation of chemical compounds in hydrofluorocarbon solvents <\/strong>S.J.P. Saul, J. Micklefield and S. Corr. <strong>2004,<\/strong>\u00a0eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:13a40\">13a40<\/a><\/li>\n<li><strong>Metabolic flux analysis for calcium dependent antibiotic (CDA) production in Streptomyces coelicolor. <\/strong>H. B. Kim, C. P. Smith, J. Micklefield and F. Mavituna. <em>Metab. Eng<\/em>. <strong>2004,<\/strong>\u00a06: 313-325. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a4603\">1a4603<\/a><\/li>\n<li><strong>NMR structure determination and calcium binding effects of lipopeptide antibiotic daptomycin. <\/strong>L.-J. Ball, C.M. Goult, J.A. Donarski, J. Micklefield and V. Ramesh. <em>Org. Biomol. Chem<\/em>. <strong>2004,<\/strong>\u00a02: 1872-1878. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a4485\">1a4485<\/a><\/li>\n<li><strong>Nucleic acid binding properties of thyminyl and adeninyl pyrrolidine-amide oligonucleotide mimics (POM).<\/strong> Tan, T. H. Samuel; Hickman, David T.; Morral, Jordi; Beadham, Ian G.; Micklefield, Jason. <em>Chem. Commun<\/em>. <strong>2004,<\/strong>\u00a0516-517. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a4486\">1a4486<\/a><\/li>\n<li><strong>Superior nucleic acid binding properties of thyminyl and adeninyl pyrrolidine-amide oligonucleotide mimics (POM).<\/strong> T.H.S. Tan, D. T. Hickman, J. Morral, I. G. Beadham and J. Micklefield. <em>Chem Commun<\/em>. <strong>2004,<\/strong>\u00a0516-517. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a5088\">1a5088<\/a><\/li>\n<\/ul>\n<p>2003<\/p>\n<ul>\n<li><strong>Design, synthesis, conformational analysis and nucleic acid hybridization properties of thymidyl pyrrolidine-amide oligonucleotide mimics (POM). <\/strong>D. T. Hickman, T. H. S. Tan, J. Morral, P. M. King, M. A. Cooper and J. Micklefield. <em>Org. Biomol. Chem<\/em>. <strong>2003,<\/strong>\u00a01: 3277-3292. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a4488\">1a4488<\/a><\/li>\n<\/ul>\n<p>2002<\/p>\n<ul>\n<li><strong>Structure, biosynthetic origin, and engineered biosynthesis of calcium-dependent antibiotics from Streptomyces coelicolor. <\/strong>Z. Hojati, C. Milne, B. Harvey, L. Gordon, M. Borg, F. Flett, B. Wilkinson, P. J. Sidebottom, B. A. M. Rudd, M. A. Hayes, C. P. Smith\u00a7 and J. Micklefield. <em>Chemistry &amp; Biology<\/em>. <strong>2002,<\/strong>\u00a09 (11): 1175-1187. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a212\">1a212<\/a>| DOI:<a href=\"http:\/\/dx.doi.org\/10.1016\/S1074-5521(02)00252-1\">10.1016\/S1074-5521(02)00252-1<\/a><\/li>\n<li><strong>Synthesis and nucleic-acid-binding properties of sulfamide- and 3&#8242;-N-sulfamate-modified DNA.<\/strong> K. J. Fettes, N. Howard, D. T. Hickman, S. A. Adah, M. R. Player, P. F. Torrence and J. Micklefield. <em>J. Chem. Soc<\/em>., Perkin Trans. <strong>2002,<\/strong>\u00a01: 485-495. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a3555\">1a3555<\/a><\/li>\n<\/ul>\n<p>2001<\/p>\n<ul>\n<li><strong>Backbone modification of nucleic acids: synthesis, structure and therapeutic applications. <\/strong>Micklefield, Jason. <em>Curr. Med. Chem<\/em>. <strong>2001,<\/strong>\u00a08: 1157-1179. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a3478\">1a3478<\/a><\/li>\n<li><strong>Kinetically selective binding of single stranded RNA over DNA by a pyrrolidine-amide oligonucleotide mimic (POM). <\/strong>D. T. Hickman, P. M. King, J. M. Slater, M. A. Cooper and J. Micklefield. <em>Nucleosides, Nucleotides &amp; Nucleic Acids<\/em>. <strong>2001,<\/strong>\u00a020: 1169-1172. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a3477\">1a3477<\/a><\/li>\n<\/ul>\n<p>2000<\/p>\n<ul>\n<li><strong>Replacement of the phosphodiester linkage in DNA with sulfamide and 3\u00b4-N-sulfamate groups. <\/strong>K. J. Fettes, N. Howard, D. T. Hickman, S. A. Adah, M. R. Player, P. F. Torrence and J. Micklefield. <em>Chem. Commun<\/em>. <strong>2000,<\/strong>\u00a0765-766. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a5820\">1a5820<\/a><\/li>\n<li><strong>Unusual RNA &amp; DNA binding properties of a novel pyrrolidine-amide oligonucleotide mimics (POM). <\/strong>D. T. Hickman, P. M. King, M. A. Cooper, J. M. Slater and J. Micklefield. <em>Chem. Commun<\/em>. <strong>2000,<\/strong>\u00a02251-2252. eScholarID:<a href=\"http:\/\/www.manchester.ac.uk\/escholar\/uk-ac-man-scw:1a5819\">1a5819<\/a><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>2025: Enzymatic Cascades for Stereoselective and Regioselective Amide Bond Assembly.\u00a0D. Torri, L. Bering, L. R. L. Yates, S.M. Angiolini, G. Xu, S. Cuesta-Hoyos, S. A. Shepherd &amp; J. Micklefield* Angew. Chem. Int. Ed. 2025, 64, e202422185 (https:\/\/doi.org\/10.1002\/anie.202422185) Integrating Enzymes with &hellip; <a href=\"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/?page_id=11\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-11","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/index.php?rest_route=\/wp\/v2\/pages\/11","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=11"}],"version-history":[{"count":76,"href":"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/index.php?rest_route=\/wp\/v2\/pages\/11\/revisions"}],"predecessor-version":[{"id":1703,"href":"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/index.php?rest_route=\/wp\/v2\/pages\/11\/revisions\/1703"}],"wp:attachment":[{"href":"https:\/\/www.micklefieldlab.chemistry.manchester.ac.uk\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=11"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}